Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A

A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging f...

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Main Authors:	Jang, Jin Woo, Kim, Ju Yeon, Yoon, Jung, Yoon, Soo Young, Cho, Chi Hyun, Han, Eun Taek, An, Seong Soo A., Lim, Chae Seung
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274835/

id	pubmed-4274835
recordtype	oai_dc
spelling	pubmed-42748352014-12-29 Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A Jang, Jin Woo Kim, Ju Yeon Yoon, Jung Yoon, Soo Young Cho, Chi Hyun Han, Eun Taek An, Seong Soo A. Lim, Chae Seung Research Article A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%). High correlation of measured parasitaemia (r = 0.98, P < 0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients. Hindawi Publishing Corporation 2014 2014-12-08 /pmc/articles/PMC4274835/ /pubmed/25548783 http://dx.doi.org/10.1155/2014/536723 Text en Copyright © 2014 Jin Woo Jang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jang, Jin Woo Kim, Ju Yeon Yoon, Jung Yoon, Soo Young Cho, Chi Hyun Han, Eun Taek An, Seong Soo A. Lim, Chae Seung
spellingShingle	Jang, Jin Woo Kim, Ju Yeon Yoon, Jung Yoon, Soo Young Cho, Chi Hyun Han, Eun Taek An, Seong Soo A. Lim, Chae Seung Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
author_facet	Jang, Jin Woo Kim, Ju Yeon Yoon, Jung Yoon, Soo Young Cho, Chi Hyun Han, Eun Taek An, Seong Soo A. Lim, Chae Seung
author_sort	Jang, Jin Woo
title	Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
title_short	Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
title_full	Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
title_fullStr	Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
title_full_unstemmed	Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A
title_sort	flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with sybr green i and cd235a
description	A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2–37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2–66.0%). High correlation of measured parasitaemia (r = 0.98, P < 0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274835/
_version_	1613170108481404928

Flow Cytometric Enumeration of Parasitemia in Cultures of Plasmodium falciparum Stained with SYBR Green I and CD235A

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