Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae

Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae

Fucoidans are matrix polysaccharides from marine brown algae, consisting of an α-l-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and het...

Full description

Bibliographic Details
Main Authors: Descamps, Valérie, Colin, Sébastien, Lahaye, Marc, Jam, Murielle, Richard, Christophe, Potin, Philippe, Barbeyron, Tristan, Yvin, Jean-Claude, Kloareg, Bernard
Format: Online
Language:English
Published: Springer-Verlag 2006
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273248/
id pubmed-4273248
recordtype oai_dc
spelling pubmed-42732482014-12-24 Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae Descamps, Valérie Colin, Sébastien Lahaye, Marc Jam, Murielle Richard, Christophe Potin, Philippe Barbeyron, Tristan Yvin, Jean-Claude Kloareg, Bernard Original Article Fucoidans are matrix polysaccharides from marine brown algae, consisting of an α-l-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and heterologous systems, no convenient sources with fucanase activity are available yet for the degradation of the fucalean algae. We here report on the isolation, characterization, and culture conditions of a bacterial strain capable of degrading various brown algal fucoidans. This bacterium, a member of the family Flavobacteriaceae, was shown to secrete fucoidan endo-hydrolase activity. An extracellular enzyme preparation was used to degrade the fucoidan from the brown alga Pelvetia canaliculata. End products included a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of α-1→3-l-fucopyranose-2-sulfate-α-1→4-l-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end. Springer-Verlag 2006-01-01 2006 /pmc/articles/PMC4273248/ /pubmed/16222488 http://dx.doi.org/10.1007/s10126-005-5107-0 Text en © Springer Science+Business Media, Inc. 2006
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Descamps, Valérie
Colin, Sébastien
Lahaye, Marc
Jam, Murielle
Richard, Christophe
Potin, Philippe
Barbeyron, Tristan
Yvin, Jean-Claude
Kloareg, Bernard
spellingShingle Descamps, Valérie
Colin, Sébastien
Lahaye, Marc
Jam, Murielle
Richard, Christophe
Potin, Philippe
Barbeyron, Tristan
Yvin, Jean-Claude
Kloareg, Bernard
Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
author_facet Descamps, Valérie
Colin, Sébastien
Lahaye, Marc
Jam, Murielle
Richard, Christophe
Potin, Philippe
Barbeyron, Tristan
Yvin, Jean-Claude
Kloareg, Bernard
author_sort Descamps, Valérie
title Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
title_short Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
title_full Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
title_fullStr Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
title_full_unstemmed Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
title_sort isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae
description Fucoidans are matrix polysaccharides from marine brown algae, consisting of an α-l-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and heterologous systems, no convenient sources with fucanase activity are available yet for the degradation of the fucalean algae. We here report on the isolation, characterization, and culture conditions of a bacterial strain capable of degrading various brown algal fucoidans. This bacterium, a member of the family Flavobacteriaceae, was shown to secrete fucoidan endo-hydrolase activity. An extracellular enzyme preparation was used to degrade the fucoidan from the brown alga Pelvetia canaliculata. End products included a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of α-1→3-l-fucopyranose-2-sulfate-α-1→4-l-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end.
publisher Springer-Verlag
publishDate 2006
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273248/
_version_ 1613169561963593728

Similar Items