Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC p...

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Main Authors:	Deng, Xiaoyu, Cong, Yanlong, Yin, Renfu, Yang, Guilian, Ding, Chan, Yu, Shengqing, Liu, Xiufan, Wang, Chunfeng, Ding, Zhuang
Format:	Online
Language:	English
Published:	The Korean Society of Veterinary Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269593/

id	pubmed-4269593
recordtype	oai_dc
spelling	pubmed-42695932014-12-19 Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus Deng, Xiaoyu Cong, Yanlong Yin, Renfu Yang, Guilian Ding, Chan Yu, Shengqing Liu, Xiufan Wang, Chunfeng Ding, Zhuang Original Article Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods. The Korean Society of Veterinary Science 2014-12 2014-12-15 /pmc/articles/PMC4269593/ /pubmed/25234324 http://dx.doi.org/10.4142/jvs.2014.15.4.511 Text en © 2014 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Deng, Xiaoyu Cong, Yanlong Yin, Renfu Yang, Guilian Ding, Chan Yu, Shengqing Liu, Xiufan Wang, Chunfeng Ding, Zhuang
spellingShingle	Deng, Xiaoyu Cong, Yanlong Yin, Renfu Yang, Guilian Ding, Chan Yu, Shengqing Liu, Xiufan Wang, Chunfeng Ding, Zhuang Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
author_facet	Deng, Xiaoyu Cong, Yanlong Yin, Renfu Yang, Guilian Ding, Chan Yu, Shengqing Liu, Xiufan Wang, Chunfeng Ding, Zhuang
author_sort	Deng, Xiaoyu
title	Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
title_short	Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
title_full	Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
title_fullStr	Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
title_full_unstemmed	Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
title_sort	proteomic analysis of chicken peripheral blood mononuclear cells after infection by newcastle disease virus
description	Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.
publisher	The Korean Society of Veterinary Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269593/
_version_	1613168499546390528

Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

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