New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712

Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916–sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does...

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Main Authors:	Fernández-Martínez, Lorena T., Borsetto, Chiara, Gomez-Escribano, Juan Pablo, Bibb, Maureen J., Al-Bassam, Mahmoud M., Chandra, Govind, Bibb, Mervyn J.
Format:	Online
Language:	English
Published:	American Society for Microbiology 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249514/

id	pubmed-4249514
recordtype	oai_dc
spelling	pubmed-42495142014-12-31 New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712 Fernández-Martínez, Lorena T. Borsetto, Chiara Gomez-Escribano, Juan Pablo Bibb, Maureen J. Al-Bassam, Mahmoud M. Chandra, Govind Bibb, Mervyn J. Chemistry; Biosynthesis Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916–sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na+/H+ antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway. American Society for Microbiology 2014-12 /pmc/articles/PMC4249514/ /pubmed/25267678 http://dx.doi.org/10.1128/AAC.04272-14 Text en Copyright © 2014 Fernández-Martínez et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Fernández-Martínez, Lorena T. Borsetto, Chiara Gomez-Escribano, Juan Pablo Bibb, Maureen J. Al-Bassam, Mahmoud M. Chandra, Govind Bibb, Mervyn J.
spellingShingle	Fernández-Martínez, Lorena T. Borsetto, Chiara Gomez-Escribano, Juan Pablo Bibb, Maureen J. Al-Bassam, Mahmoud M. Chandra, Govind Bibb, Mervyn J. New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
author_facet	Fernández-Martínez, Lorena T. Borsetto, Chiara Gomez-Escribano, Juan Pablo Bibb, Maureen J. Al-Bassam, Mahmoud M. Chandra, Govind Bibb, Mervyn J.
author_sort	Fernández-Martínez, Lorena T.
title	New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
title_short	New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
title_full	New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
title_fullStr	New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
title_full_unstemmed	New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712
title_sort	new insights into chloramphenicol biosynthesis in streptomyces venezuelae atcc 10712
description	Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916–sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na+/H+ antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.
publisher	American Society for Microbiology
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249514/
_version_	1613162427070808064

New Insights into Chloramphenicol Biosynthesis in Streptomyces venezuelae ATCC 10712

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