Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy

Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the enti...

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Main Authors:	Ayaz, Sultan, Ullah, Riaz, AbdEl-Salam, Naser M., Shams, Sumiara, Niaz, Sadaf
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248420/

id	pubmed-4248420
recordtype	oai_dc
spelling	pubmed-42484202014-12-07 Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy Ayaz, Sultan Ullah, Riaz AbdEl-Salam, Naser M. Shams, Sumiara Niaz, Sadaf Research Article Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy. Hindawi Publishing Corporation 2014 2014-11-13 /pmc/articles/PMC4248420/ /pubmed/25485297 http://dx.doi.org/10.1155/2014/462084 Text en Copyright © 2014 Sultan Ayaz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ayaz, Sultan Ullah, Riaz AbdEl-Salam, Naser M. Shams, Sumiara Niaz, Sadaf
spellingShingle	Ayaz, Sultan Ullah, Riaz AbdEl-Salam, Naser M. Shams, Sumiara Niaz, Sadaf Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
author_facet	Ayaz, Sultan Ullah, Riaz AbdEl-Salam, Naser M. Shams, Sumiara Niaz, Sadaf
author_sort	Ayaz, Sultan
title	Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
title_short	Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
title_full	Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
title_fullStr	Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
title_full_unstemmed	Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy
title_sort	fasciola hepatica in some buffaloes and cattle by pcr and microscopy
description	Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248420/
_version_	1613162269880877056

Fasciola hepatica in Some Buffaloes and Cattle by PCR and Microscopy

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