Regulation of angiopoietin‐like protein 4 production during and after exercise
Angiopoietin‐like protein 4 (ANGPTL4) may regulate lipoprotein lipase‐dependent plasma clearance of triacylglycerol from skeletal muscle during exercise. The aim of this study was to examine the importance of muscle in regulating ANGPTL4 in response to exercise. We sampled muscle biopsies and serum...
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| Format: | Online |
| Language: | English |
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Wiley Periodicals, Inc.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246580/ |
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pubmed-4246580 |
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pubmed-42465802014-12-18 Regulation of angiopoietin‐like protein 4 production during and after exercise Norheim, Frode Hjorth, Marit Langleite, Torgrim M. Lee, Sindre Holen, Torgeir Bindesbøll, Christian Stadheim, Hans K. Gulseth, Hanne L. Birkeland, Kåre I. Kielland, Anders Jensen, Jørgen Dalen, Knut T. Drevon, Christian A. Original Research Angiopoietin‐like protein 4 (ANGPTL4) may regulate lipoprotein lipase‐dependent plasma clearance of triacylglycerol from skeletal muscle during exercise. The aim of this study was to examine the importance of muscle in regulating ANGPTL4 in response to exercise. We sampled muscle biopsies and serum before, immediately after, and 2 h after 45 min of ergometer cycling. Sampling was done before and after a 12‐week training intervention in controls and dysglycemic subjects. Moreover, fat biopsies were taken before and after the training intervention. The regulation of ANGPTL4 was also investigated in several tissues of exercising mice, and in cultured myotubes. ANGPTL4 levels in serum and expression in muscle were highest 2 h after exercise in both groups. Whereas ANGPTL4 was higher in muscle of exercising controls as compared to dysglycemic subjects, the opposite was observed in serum. In exercising mice, Angptl4 mRNA showed both higher basal expression and induction in liver compared to muscle. Angptl4 mRNA was much higher in adipose tissue than muscle and was also induced by exercise. We observed two mRNA isoforms of ANGPTL4 in muscle and fat in humans. Both were induced by exercise in muscle; one isoform was expressed 5‐ to 10‐fold higher than the other. Studies in mice and cultured myotubes showed that both fatty acids and cortisol have the potential to increase ANGPTL4 expression in muscle during exercise. In conclusion, ANGPTL4 is markedly induced in muscle in response to exercise. However, liver and adipose tissue may contribute more than muscle to the exercise‐induced increase in circulating ANGPTL4. Wiley Periodicals, Inc. 2014-08-19 /pmc/articles/PMC4246580/ /pubmed/25138789 http://dx.doi.org/10.14814/phy2.12109 Text en © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Norheim, Frode Hjorth, Marit Langleite, Torgrim M. Lee, Sindre Holen, Torgeir Bindesbøll, Christian Stadheim, Hans K. Gulseth, Hanne L. Birkeland, Kåre I. Kielland, Anders Jensen, Jørgen Dalen, Knut T. Drevon, Christian A. |
| spellingShingle |
Norheim, Frode Hjorth, Marit Langleite, Torgrim M. Lee, Sindre Holen, Torgeir Bindesbøll, Christian Stadheim, Hans K. Gulseth, Hanne L. Birkeland, Kåre I. Kielland, Anders Jensen, Jørgen Dalen, Knut T. Drevon, Christian A. Regulation of angiopoietin‐like protein 4 production during and after exercise |
| author_facet |
Norheim, Frode Hjorth, Marit Langleite, Torgrim M. Lee, Sindre Holen, Torgeir Bindesbøll, Christian Stadheim, Hans K. Gulseth, Hanne L. Birkeland, Kåre I. Kielland, Anders Jensen, Jørgen Dalen, Knut T. Drevon, Christian A. |
| author_sort |
Norheim, Frode |
| title |
Regulation of angiopoietin‐like protein 4 production during and after exercise |
| title_short |
Regulation of angiopoietin‐like protein 4 production during and after exercise |
| title_full |
Regulation of angiopoietin‐like protein 4 production during and after exercise |
| title_fullStr |
Regulation of angiopoietin‐like protein 4 production during and after exercise |
| title_full_unstemmed |
Regulation of angiopoietin‐like protein 4 production during and after exercise |
| title_sort |
regulation of angiopoietin‐like protein 4 production during and after exercise |
| description |
Angiopoietin‐like protein 4 (ANGPTL4) may regulate lipoprotein lipase‐dependent plasma clearance of triacylglycerol from skeletal muscle during exercise. The aim of this study was to examine the importance of muscle in regulating ANGPTL4 in response to exercise. We sampled muscle biopsies and serum before, immediately after, and 2 h after 45 min of ergometer cycling. Sampling was done before and after a 12‐week training intervention in controls and dysglycemic subjects. Moreover, fat biopsies were taken before and after the training intervention. The regulation of ANGPTL4 was also investigated in several tissues of exercising mice, and in cultured myotubes. ANGPTL4 levels in serum and expression in muscle were highest 2 h after exercise in both groups. Whereas ANGPTL4 was higher in muscle of exercising controls as compared to dysglycemic subjects, the opposite was observed in serum. In exercising mice, Angptl4 mRNA showed both higher basal expression and induction in liver compared to muscle. Angptl4 mRNA was much higher in adipose tissue than muscle and was also induced by exercise. We observed two mRNA isoforms of ANGPTL4 in muscle and fat in humans. Both were induced by exercise in muscle; one isoform was expressed 5‐ to 10‐fold higher than the other. Studies in mice and cultured myotubes showed that both fatty acids and cortisol have the potential to increase ANGPTL4 expression in muscle during exercise. In conclusion, ANGPTL4 is markedly induced in muscle in response to exercise. However, liver and adipose tissue may contribute more than muscle to the exercise‐induced increase in circulating ANGPTL4. |
| publisher |
Wiley Periodicals, Inc. |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246580/ |
| _version_ |
1613161657513541632 |