1H-NMR metabolite profiles of different strains of Plasmodium falciparum

Although efforts to understand the basis for inter-strain phenotypic variation in the most virulent malaria species, Plasmodium falciparum, have benefited from advances in genomic technologies, there have to date been few metabolomic studies of this parasite. Using 1H-NMR spectroscopy, we have compa...

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Main Authors:	Teng, Rongwei, Lehane, Adele M., Winterberg, Markus, Shafik, Sarah H., Summers, Robert L., Martin, Rowena E., van Schalkwyk, Donelly A., Junankar, Pauline R., Kirk, Kiaran
Format:	Online
Language:	English
Published:	Portland Press Ltd. 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240024/

id	pubmed-4240024
recordtype	oai_dc
spelling	pubmed-42400242014-12-01 1H-NMR metabolite profiles of different strains of Plasmodium falciparum Teng, Rongwei Lehane, Adele M. Winterberg, Markus Shafik, Sarah H. Summers, Robert L. Martin, Rowena E. van Schalkwyk, Donelly A. Junankar, Pauline R. Kirk, Kiaran Original Paper Although efforts to understand the basis for inter-strain phenotypic variation in the most virulent malaria species, Plasmodium falciparum, have benefited from advances in genomic technologies, there have to date been few metabolomic studies of this parasite. Using 1H-NMR spectroscopy, we have compared the metabolite profiles of red blood cells infected with different P. falciparum strains. These included both chloroquine-sensitive and chloroquine-resistant strains, as well as transfectant lines engineered to express different isoforms of the chloroquine-resistance-conferring pfcrt (P. falciparum chloroquine resistance transporter). Our analyses revealed strain-specific differences in a range of metabolites. There was marked variation in the levels of the membrane precursors choline and phosphocholine, with some strains having >30-fold higher choline levels and >5-fold higher phosphocholine levels than others. Chloroquine-resistant strains showed elevated levels of a number of amino acids relative to chloroquine-sensitive strains, including an approximately 2-fold increase in aspartate levels. The elevation in amino acid levels was attributable to mutations in pfcrt. Pfcrt-linked differences in amino acid abundance were confirmed using alternate extraction and detection (HPLC) methods. Mutations acquired to withstand chloroquine exposure therefore give rise to significant biochemical alterations in the parasite. Portland Press Ltd. 2014-11-21 /pmc/articles/PMC4240024/ /pubmed/25405893 http://dx.doi.org/10.1042/BSR20140134 Text en © 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Teng, Rongwei Lehane, Adele M. Winterberg, Markus Shafik, Sarah H. Summers, Robert L. Martin, Rowena E. van Schalkwyk, Donelly A. Junankar, Pauline R. Kirk, Kiaran
spellingShingle	Teng, Rongwei Lehane, Adele M. Winterberg, Markus Shafik, Sarah H. Summers, Robert L. Martin, Rowena E. van Schalkwyk, Donelly A. Junankar, Pauline R. Kirk, Kiaran 1H-NMR metabolite profiles of different strains of Plasmodium falciparum
author_facet	Teng, Rongwei Lehane, Adele M. Winterberg, Markus Shafik, Sarah H. Summers, Robert L. Martin, Rowena E. van Schalkwyk, Donelly A. Junankar, Pauline R. Kirk, Kiaran
author_sort	Teng, Rongwei
title	1H-NMR metabolite profiles of different strains of Plasmodium falciparum
title_short	1H-NMR metabolite profiles of different strains of Plasmodium falciparum
title_full	1H-NMR metabolite profiles of different strains of Plasmodium falciparum
title_fullStr	1H-NMR metabolite profiles of different strains of Plasmodium falciparum
title_full_unstemmed	1H-NMR metabolite profiles of different strains of Plasmodium falciparum
title_sort	1h-nmr metabolite profiles of different strains of plasmodium falciparum
description	Although efforts to understand the basis for inter-strain phenotypic variation in the most virulent malaria species, Plasmodium falciparum, have benefited from advances in genomic technologies, there have to date been few metabolomic studies of this parasite. Using 1H-NMR spectroscopy, we have compared the metabolite profiles of red blood cells infected with different P. falciparum strains. These included both chloroquine-sensitive and chloroquine-resistant strains, as well as transfectant lines engineered to express different isoforms of the chloroquine-resistance-conferring pfcrt (P. falciparum chloroquine resistance transporter). Our analyses revealed strain-specific differences in a range of metabolites. There was marked variation in the levels of the membrane precursors choline and phosphocholine, with some strains having >30-fold higher choline levels and >5-fold higher phosphocholine levels than others. Chloroquine-resistant strains showed elevated levels of a number of amino acids relative to chloroquine-sensitive strains, including an approximately 2-fold increase in aspartate levels. The elevation in amino acid levels was attributable to mutations in pfcrt. Pfcrt-linked differences in amino acid abundance were confirmed using alternate extraction and detection (HPLC) methods. Mutations acquired to withstand chloroquine exposure therefore give rise to significant biochemical alterations in the parasite.
publisher	Portland Press Ltd.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240024/
_version_	1613159503345221632

1H-NMR metabolite profiles of different strains of Plasmodium falciparum

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