Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)

The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or dec...

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Main Authors:	Lin-Wang, Kui, McGhie, Tony K., Wang, Mindy, Liu, Yuhui, Warren, Benjamin, Storey, Roy, Espley, Richard V., Allan, Andrew C.
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237049/

id	pubmed-4237049
recordtype	oai_dc
spelling	pubmed-42370492014-12-04 Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) Lin-Wang, Kui McGhie, Tony K. Wang, Mindy Liu, Yuhui Warren, Benjamin Storey, Roy Espley, Richard V. Allan, Andrew C. Plant Science The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33) did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin, and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH, and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10's activation of biosynthetic steps is inhibited by the strawberry repressor MYB1. Frontiers Media S.A. 2014-11-19 /pmc/articles/PMC4237049/ /pubmed/25477896 http://dx.doi.org/10.3389/fpls.2014.00651 Text en Copyright © 2014 Lin-Wang, McGhie, Wang, Liu, Warren, Storey, Espley and Allan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lin-Wang, Kui McGhie, Tony K. Wang, Mindy Liu, Yuhui Warren, Benjamin Storey, Roy Espley, Richard V. Allan, Andrew C.
spellingShingle	Lin-Wang, Kui McGhie, Tony K. Wang, Mindy Liu, Yuhui Warren, Benjamin Storey, Roy Espley, Richard V. Allan, Andrew C. Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
author_facet	Lin-Wang, Kui McGhie, Tony K. Wang, Mindy Liu, Yuhui Warren, Benjamin Storey, Roy Espley, Richard V. Allan, Andrew C.
author_sort	Lin-Wang, Kui
title	Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
title_short	Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
title_full	Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
title_fullStr	Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
title_full_unstemmed	Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
title_sort	engineering the anthocyanin regulatory complex of strawberry (fragaria vesca)
description	The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33) did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin, and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH, and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10's activation of biosynthetic steps is inhibited by the strawberry repressor MYB1.
publisher	Frontiers Media S.A.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237049/
_version_	1613158542151254016

Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)

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