Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress

Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress

Misfolding and aggregation into amyloids of the prion protein (PrP) is responsible for the development of fatal transmissible neurodegenerative diseases. Various studies on curcumin demonstrate promise for the prevention of Alzheimer’s disease and inhibition of PrPres accumulation. To evaluate the e...

Full description

Bibliographic Details
Main Authors: Lin, Chi-Fen, Yu, Kun-Hua, Jheng, Cheng-Ping, Chung, Raymond, Lee, Cheng-I
Format: Online
Language:English
Published: MDPI 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235698/
id pubmed-4235698
recordtype oai_dc
spelling pubmed-42356982014-11-25 Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress Lin, Chi-Fen Yu, Kun-Hua Jheng, Cheng-Ping Chung, Raymond Lee, Cheng-I Article Misfolding and aggregation into amyloids of the prion protein (PrP) is responsible for the development of fatal transmissible neurodegenerative diseases. Various studies on curcumin demonstrate promise for the prevention of Alzheimer’s disease and inhibition of PrPres accumulation. To evaluate the effect of curcumin on amyloid fibrillation of prion protein, we first investigated the effect of curcumin on mouse prion protein (mPrP) in a cell-free system. Curcumin reduced the prion fibril formation significantly. Furthermore, we monitored the change in apoptosis and reactive oxygen species (ROS) level upon curcumin treatment in mouse neuroblastoma cells (N2a). Curcumin effectively rescues the cells from apoptosis and decreases the ROS level caused by subsequent co-incubation with prion amyloid fibrils. The assays in cell-free mPrP and in N2a cells of this work verified the promising effect of curcumin on the prevention of transmissible neurodegenerative diseases. MDPI 2013-07-25 /pmc/articles/PMC4235698/ /pubmed/25437204 http://dx.doi.org/10.3390/pathogens2030506 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Lin, Chi-Fen
Yu, Kun-Hua
Jheng, Cheng-Ping
Chung, Raymond
Lee, Cheng-I
spellingShingle Lin, Chi-Fen
Yu, Kun-Hua
Jheng, Cheng-Ping
Chung, Raymond
Lee, Cheng-I
Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
author_facet Lin, Chi-Fen
Yu, Kun-Hua
Jheng, Cheng-Ping
Chung, Raymond
Lee, Cheng-I
author_sort Lin, Chi-Fen
title Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
title_short Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
title_full Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
title_fullStr Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
title_full_unstemmed Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
title_sort curcumin reduces amyloid fibrillation of prion protein and decreases reactive oxidative stress
description Misfolding and aggregation into amyloids of the prion protein (PrP) is responsible for the development of fatal transmissible neurodegenerative diseases. Various studies on curcumin demonstrate promise for the prevention of Alzheimer’s disease and inhibition of PrPres accumulation. To evaluate the effect of curcumin on amyloid fibrillation of prion protein, we first investigated the effect of curcumin on mouse prion protein (mPrP) in a cell-free system. Curcumin reduced the prion fibril formation significantly. Furthermore, we monitored the change in apoptosis and reactive oxygen species (ROS) level upon curcumin treatment in mouse neuroblastoma cells (N2a). Curcumin effectively rescues the cells from apoptosis and decreases the ROS level caused by subsequent co-incubation with prion amyloid fibrils. The assays in cell-free mPrP and in N2a cells of this work verified the promising effect of curcumin on the prevention of transmissible neurodegenerative diseases.
publisher MDPI
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235698/
_version_ 1613157999063334912

Similar Items