Effect of Melatonin on Human Dental Papilla Cells
Melatonin regulates a variety of biological processes, which are the control of circadian rhythms, regulation of seasonal reproductive function and body temperature, free radical scavenging and so on. Our previous studies have shown that various cells exist in human and mouse tooth germs that expres...
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| Format: | Online |
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MDPI
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227163/ |
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pubmed-42271632014-11-12 Effect of Melatonin on Human Dental Papilla Cells Tachibana, Ryusuke Tatehara, Seiko Kumasaka, Shuku Tokuyama, Reiko Satomura, Kazuhito Article Melatonin regulates a variety of biological processes, which are the control of circadian rhythms, regulation of seasonal reproductive function and body temperature, free radical scavenging and so on. Our previous studies have shown that various cells exist in human and mouse tooth germs that express the melatonin 1a receptor (Mel1aR). However, little is known about the effects of melatonin on tooth development and growth. The present study was performed to examine the possibility that melatonin might exert its influence on tooth development. DP-805 cells, a human dental papilla cell line, were shown to express Mel1aR. Expression levels of mRNA for Mel1aR in DP-805 cells increased until 3 days after reaching confluence and decreased thereafter. Real-time reverse transcription-polymerase chain reaction showed that melatonin increased the expression of mRNAs for osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotin (DSPP). Melatonin also enhanced the mineralized matrix formation in DP-805 cell cultures in a dose-dependent manner. These results strongly suggest that melatonin may play a physiological role in tooth development/growth by regulating the cellular function of odontogenic cells in tooth germs. MDPI 2014-09-26 /pmc/articles/PMC4227163/ /pubmed/25264744 http://dx.doi.org/10.3390/ijms151017304 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Tachibana, Ryusuke Tatehara, Seiko Kumasaka, Shuku Tokuyama, Reiko Satomura, Kazuhito |
| spellingShingle |
Tachibana, Ryusuke Tatehara, Seiko Kumasaka, Shuku Tokuyama, Reiko Satomura, Kazuhito Effect of Melatonin on Human Dental Papilla Cells |
| author_facet |
Tachibana, Ryusuke Tatehara, Seiko Kumasaka, Shuku Tokuyama, Reiko Satomura, Kazuhito |
| author_sort |
Tachibana, Ryusuke |
| title |
Effect of Melatonin on Human Dental Papilla Cells |
| title_short |
Effect of Melatonin on Human Dental Papilla Cells |
| title_full |
Effect of Melatonin on Human Dental Papilla Cells |
| title_fullStr |
Effect of Melatonin on Human Dental Papilla Cells |
| title_full_unstemmed |
Effect of Melatonin on Human Dental Papilla Cells |
| title_sort |
effect of melatonin on human dental papilla cells |
| description |
Melatonin regulates a variety of biological processes, which are the control of circadian rhythms, regulation of seasonal reproductive function and body temperature, free radical scavenging and so on. Our previous studies have shown that various cells exist in human and mouse tooth germs that express the melatonin 1a receptor (Mel1aR). However, little is known about the effects of melatonin on tooth development and growth. The present study was performed to examine the possibility that melatonin might exert its influence on tooth development. DP-805 cells, a human dental papilla cell line, were shown to express Mel1aR. Expression levels of mRNA for Mel1aR in DP-805 cells increased until 3 days after reaching confluence and decreased thereafter. Real-time reverse transcription-polymerase chain reaction showed that melatonin increased the expression of mRNAs for osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotin (DSPP). Melatonin also enhanced the mineralized matrix formation in DP-805 cell cultures in a dose-dependent manner. These results strongly suggest that melatonin may play a physiological role in tooth development/growth by regulating the cellular function of odontogenic cells in tooth germs. |
| publisher |
MDPI |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227163/ |
| _version_ |
1613154758632144896 |