Non-destructive on-chip cell sorting system with real-time microscopic image processing

Studying cell functions for cellomics studies often requires the use of purified individual cells from mixtures of various kinds of cells. We have developed a new non-destructive on-chip cell sorting system for single cell based cultivation, by exploiting the advantage of microfluidics and electrost...

Full description

Bibliographic Details
Main Authors:	Takahashi, Kazunori, Hattori, Akihiro, Suzuki, Ikurou, Ichiki, Takanori, Yasuda, Kenji
Format:	Online
Language:	English
Published:	BioMed Central 2004
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC421746/

id	pubmed-421746
recordtype	oai_dc
spelling	pubmed-4217462004-06-13 Non-destructive on-chip cell sorting system with real-time microscopic image processing Takahashi, Kazunori Hattori, Akihiro Suzuki, Ikurou Ichiki, Takanori Yasuda, Kenji Research Studying cell functions for cellomics studies often requires the use of purified individual cells from mixtures of various kinds of cells. We have developed a new non-destructive on-chip cell sorting system for single cell based cultivation, by exploiting the advantage of microfluidics and electrostatic force. The system consists of the following two parts: a cell sorting chip made of poly-dimethylsiloxane (PDMS) on a 0.2-mm-thick glass slide, and an image analysis system with a phase-contrast/fluorescence microscope. The unique features of our system include (i) identification of a target from sample cells is achieved by comparison of the 0.2-μm-resolution phase-contrast and fluorescence images of cells in the microchannel every 1/30 s; (ii) non-destructive sorting of target cells in a laminar flow by application of electrostatic repulsion force for removing unrequited cells from the one laminar flow to the other; (iii) the use of agar gel for electrodes in order to minimize the effect on cells by electrochemical reactions of electrodes, and (iv) pre-filter, which was fabricated within the channel for removal of dust contained in a sample solution from tissue extracts. The sorting chip is capable of continuous operation and we have purified more than ten thousand cells for cultivation without damaging them. Our design has proved to be very efficient and suitable for the routine use in cell purification experiments. BioMed Central 2004-06-03 /pmc/articles/PMC421746/ /pubmed/15176978 http://dx.doi.org/10.1186/1477-3155-2-5 Text en Copyright © 2004 Takahashi et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Takahashi, Kazunori Hattori, Akihiro Suzuki, Ikurou Ichiki, Takanori Yasuda, Kenji
spellingShingle	Takahashi, Kazunori Hattori, Akihiro Suzuki, Ikurou Ichiki, Takanori Yasuda, Kenji Non-destructive on-chip cell sorting system with real-time microscopic image processing
author_facet	Takahashi, Kazunori Hattori, Akihiro Suzuki, Ikurou Ichiki, Takanori Yasuda, Kenji
author_sort	Takahashi, Kazunori
title	Non-destructive on-chip cell sorting system with real-time microscopic image processing
title_short	Non-destructive on-chip cell sorting system with real-time microscopic image processing
title_full	Non-destructive on-chip cell sorting system with real-time microscopic image processing
title_fullStr	Non-destructive on-chip cell sorting system with real-time microscopic image processing
title_full_unstemmed	Non-destructive on-chip cell sorting system with real-time microscopic image processing
title_sort	non-destructive on-chip cell sorting system with real-time microscopic image processing
description	Studying cell functions for cellomics studies often requires the use of purified individual cells from mixtures of various kinds of cells. We have developed a new non-destructive on-chip cell sorting system for single cell based cultivation, by exploiting the advantage of microfluidics and electrostatic force. The system consists of the following two parts: a cell sorting chip made of poly-dimethylsiloxane (PDMS) on a 0.2-mm-thick glass slide, and an image analysis system with a phase-contrast/fluorescence microscope. The unique features of our system include (i) identification of a target from sample cells is achieved by comparison of the 0.2-μm-resolution phase-contrast and fluorescence images of cells in the microchannel every 1/30 s; (ii) non-destructive sorting of target cells in a laminar flow by application of electrostatic repulsion force for removing unrequited cells from the one laminar flow to the other; (iii) the use of agar gel for electrodes in order to minimize the effect on cells by electrochemical reactions of electrodes, and (iv) pre-filter, which was fabricated within the channel for removal of dust contained in a sample solution from tissue extracts. The sorting chip is capable of continuous operation and we have purified more than ten thousand cells for cultivation without damaging them. Our design has proved to be very efficient and suitable for the routine use in cell purification experiments.
publisher	BioMed Central
publishDate	2004
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC421746/
_version_	1611368723785973760

Non-destructive on-chip cell sorting system with real-time microscopic image processing

Similar Items