Development of Strand-Specific Real-Time RT-PCR to Distinguish Viral RNAs during Newcastle Disease Virus Infection

Newcastle disease virus (NDV) causes large losses in the global fowl industry. To better understand NDV replication and transcription cycle, quantitative detection methods for distinguishing NDV genomic RNA (gRNA), antigenomic RNA (cRNA), and messenger RNA (mRNA) in NDV-infected cells are indispensi...

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Bibliographic Details
Main Authors: Qiu, Xusheng, Yu, Yang, Yu, Shengqing, Zhan, Yuan, Wei, Nana, Song, Cuiping, Sun, Yingjie, Tan, Lei, Ding, Chan
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212552/