In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe

Renanthera imschootiana Rolfe is an endangered tropical epiphytic orchid that is threatened with extinction due to over-collection and the loss of suitable habitats. In vitro propagation is a useful way to mass produce plants for re-establishment in the wild and for commercial propagation. Seeds co...

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Main Authors:	Wu, Kunlin, Zeng, Songjun, Lin, Danni, Teixeira da Silva, Jaime A., Bu, Zhaoyang, Zhang, Jianxia, Duan, Jun
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211678/

id	pubmed-4211678
recordtype	oai_dc
spelling	pubmed-42116782014-11-05 In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe Wu, Kunlin Zeng, Songjun Lin, Danni Teixeira da Silva, Jaime A. Bu, Zhaoyang Zhang, Jianxia Duan, Jun Research Article Renanthera imschootiana Rolfe is an endangered tropical epiphytic orchid that is threatened with extinction due to over-collection and the loss of suitable habitats. In vitro propagation is a useful way to mass produce plants for re-establishment in the wild and for commercial propagation. Seeds collected 150 days after pollination (DAP) were the optimum stage for in vitro culture. Seed germination reached 93.1% on quarter-strength MS (i.e., MS containing a quarter of macro- and micronutrients) medium containing 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 20% coconut water (CW), 1.0 g l−1 peptone, 10 g l−1 sucrose and 1.0 g l−1 activated charcoal (AC). Quarter-strength MS medium supplemented with 1.0 mg l−1 BA, 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 10 g l−1 sucrose and 20% CW was suitable for the sub-culture of protocorm-like bodies (PLBs) in which the PLB proliferation ratio was 2.88. Quarter-strength MS medium containing 1.0 mg l−1 NAA, 1.0 g l−1 peptone, 100 g l−1 banana homogenate (BH), and 1.0 g l−1 AC was suitable for plantlet formation and 95.67% of plantlets developed from PLBs within 60 days of culture. Hyponex N016 medium supplemented with 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 20 g l−1 sucrose, 150 g l−1 BH, and 1.0 g l−1 AC was suitable for the in vitro growth of plantlets about 2-cm in height. Plantlets 3-cm in height or taller were transplanted to Chilean sphagnum moss, and 95% of plantlets survived after 60 days in a greenhouse. Three hundred transplanted of seedlings 360-days old were reintroduced into three natural habitats. Highest percentage survival (79.67%) was observed in Yuanjiang Nature Reserve two years after reintroduction, followed by Huolu Mountain forest park (71.33%). This protocol is an efficient means for the large-scale propagation and in vitro and in vivo germplasm conservation of R. imschootiana. Public Library of Science 2014-10-28 /pmc/articles/PMC4211678/ /pubmed/25350549 http://dx.doi.org/10.1371/journal.pone.0110033 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wu, Kunlin Zeng, Songjun Lin, Danni Teixeira da Silva, Jaime A. Bu, Zhaoyang Zhang, Jianxia Duan, Jun
spellingShingle	Wu, Kunlin Zeng, Songjun Lin, Danni Teixeira da Silva, Jaime A. Bu, Zhaoyang Zhang, Jianxia Duan, Jun In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
author_facet	Wu, Kunlin Zeng, Songjun Lin, Danni Teixeira da Silva, Jaime A. Bu, Zhaoyang Zhang, Jianxia Duan, Jun
author_sort	Wu, Kunlin
title	In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
title_short	In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
title_full	In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
title_fullStr	In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
title_full_unstemmed	In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe
title_sort	in vitro propagation and reintroduction of the endangered renanthera imschootiana rolfe
description	Renanthera imschootiana Rolfe is an endangered tropical epiphytic orchid that is threatened with extinction due to over-collection and the loss of suitable habitats. In vitro propagation is a useful way to mass produce plants for re-establishment in the wild and for commercial propagation. Seeds collected 150 days after pollination (DAP) were the optimum stage for in vitro culture. Seed germination reached 93.1% on quarter-strength MS (i.e., MS containing a quarter of macro- and micronutrients) medium containing 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 20% coconut water (CW), 1.0 g l−1 peptone, 10 g l−1 sucrose and 1.0 g l−1 activated charcoal (AC). Quarter-strength MS medium supplemented with 1.0 mg l−1 BA, 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 10 g l−1 sucrose and 20% CW was suitable for the sub-culture of protocorm-like bodies (PLBs) in which the PLB proliferation ratio was 2.88. Quarter-strength MS medium containing 1.0 mg l−1 NAA, 1.0 g l−1 peptone, 100 g l−1 banana homogenate (BH), and 1.0 g l−1 AC was suitable for plantlet formation and 95.67% of plantlets developed from PLBs within 60 days of culture. Hyponex N016 medium supplemented with 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 20 g l−1 sucrose, 150 g l−1 BH, and 1.0 g l−1 AC was suitable for the in vitro growth of plantlets about 2-cm in height. Plantlets 3-cm in height or taller were transplanted to Chilean sphagnum moss, and 95% of plantlets survived after 60 days in a greenhouse. Three hundred transplanted of seedlings 360-days old were reintroduced into three natural habitats. Highest percentage survival (79.67%) was observed in Yuanjiang Nature Reserve two years after reintroduction, followed by Huolu Mountain forest park (71.33%). This protocol is an efficient means for the large-scale propagation and in vitro and in vivo germplasm conservation of R. imschootiana.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211678/
_version_	1613149623038246912

In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe

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