Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda
In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest™ Malaria Pf/Pan Ag is one of the most commonly used malaria RDTs in several countries, includin...
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| Format: | Online |
| Language: | English |
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The Korean Society for Parasitology and Tropical Medicine
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210732/ |
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pubmed-4210732 |
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pubmed-42107322014-10-28 Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda Chong, Chom-Kyu Cho, Pyo Yun Na, Byoung-Kuk Ahn, Seong Kyu Kim, Jin Su Lee, Jin-Soo Lee, Sung-Keun Han, Eun-Taek Kim, Hak-Yong Park, Yun-Kyu Cha, Seok Ho Kim, Tong-Soo Original Article In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest™ Malaria Pf/Pan Ag is one of the most commonly used malaria RDTs in several countries, including Korea and India. In this study, we tested the diagnostic performance of this RDT in Uganda to evaluate its usefulness for field diagnosis of malaria in this country. Microscopic and PCR analyses, and the Asan EasyTest™ Malaria Pf/Pan Ag rapid diagnostic test, were performed on blood samples from 185 individuals with suspected malaria in several villages in Uganda. Compared to the microscopic analysis, the sensitivity of the RDT to detect malaria infection was 95.8% and 83.3% for Plasmodium falciparum and non-P. falciparum, respectively. Although the diagnostic sensitivity of the RDT decreased when parasitemia was ≤500 parasites/µl, it showed 96.8% sensitivity (98.4% for P. falciparum and 93.8% for non-P. falciparum) in blood samples with parasitemia ≥100 parasites/µl. The specificity of the RDT was 97.3% for P. falciparum and 97.3% for non-P. falciparum. These results collectively suggest that the accuracy of the Asan EasyTest™ Malaria Pf/Pan Ag makes it an effective point-of-care diagnostic tool for malaria in Uganda. The Korean Society for Parasitology and Tropical Medicine 2014-10 2014-10-22 /pmc/articles/PMC4210732/ /pubmed/25352698 http://dx.doi.org/10.3347/kjp.2014.52.5.501 Text en © 2014, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Chong, Chom-Kyu Cho, Pyo Yun Na, Byoung-Kuk Ahn, Seong Kyu Kim, Jin Su Lee, Jin-Soo Lee, Sung-Keun Han, Eun-Taek Kim, Hak-Yong Park, Yun-Kyu Cha, Seok Ho Kim, Tong-Soo |
| spellingShingle |
Chong, Chom-Kyu Cho, Pyo Yun Na, Byoung-Kuk Ahn, Seong Kyu Kim, Jin Su Lee, Jin-Soo Lee, Sung-Keun Han, Eun-Taek Kim, Hak-Yong Park, Yun-Kyu Cha, Seok Ho Kim, Tong-Soo Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| author_facet |
Chong, Chom-Kyu Cho, Pyo Yun Na, Byoung-Kuk Ahn, Seong Kyu Kim, Jin Su Lee, Jin-Soo Lee, Sung-Keun Han, Eun-Taek Kim, Hak-Yong Park, Yun-Kyu Cha, Seok Ho Kim, Tong-Soo |
| author_sort |
Chong, Chom-Kyu |
| title |
Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| title_short |
Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| title_full |
Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| title_fullStr |
Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| title_full_unstemmed |
Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda |
| title_sort |
evaluation of the accuracy of the easytest™ malaria pf/pan ag, a rapid diagnostic test, in uganda |
| description |
In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest™ Malaria Pf/Pan Ag is one of the most commonly used malaria RDTs in several countries, including Korea and India. In this study, we tested the diagnostic performance of this RDT in Uganda to evaluate its usefulness for field diagnosis of malaria in this country. Microscopic and PCR analyses, and the Asan EasyTest™ Malaria Pf/Pan Ag rapid diagnostic test, were performed on blood samples from 185 individuals with suspected malaria in several villages in Uganda. Compared to the microscopic analysis, the sensitivity of the RDT to detect malaria infection was 95.8% and 83.3% for Plasmodium falciparum and non-P. falciparum, respectively. Although the diagnostic sensitivity of the RDT decreased when parasitemia was ≤500 parasites/µl, it showed 96.8% sensitivity (98.4% for P. falciparum and 93.8% for non-P. falciparum) in blood samples with parasitemia ≥100 parasites/µl. The specificity of the RDT was 97.3% for P. falciparum and 97.3% for non-P. falciparum. These results collectively suggest that the accuracy of the Asan EasyTest™ Malaria Pf/Pan Ag makes it an effective point-of-care diagnostic tool for malaria in Uganda. |
| publisher |
The Korean Society for Parasitology and Tropical Medicine |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210732/ |
| _version_ |
1613149207159373824 |