An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit

A series of clinical isolates of drug-resistant (DR) Acinetobacter baumannii with diverse drug susceptibility was detected from eight patients in the emergency intensive care unit of Tokai University Hospital. The initial isolate was obtained in March 2010 (A. baumannii Tokai strain 1); subsequently...

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Main Authors:	Asai, Satomi, Umezawa, Kazuo, Iwashita, Hideo, Ohshima, Toshio, Ohashi, Maya, Sasaki, Mika, Hayashi, Hideki, Matsui, Mari, Shibayama, Keigo, Inokuchi, Sadaki, Miyachi, Hayato
Format:	Online
Language:	English
Published:	Society for General Microbiology 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209737/

id	pubmed-4209737
recordtype	oai_dc
spelling	pubmed-42097372014-11-10 An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit Asai, Satomi Umezawa, Kazuo Iwashita, Hideo Ohshima, Toshio Ohashi, Maya Sasaki, Mika Hayashi, Hideki Matsui, Mari Shibayama, Keigo Inokuchi, Sadaki Miyachi, Hayato Clinical Microbiology and Virology A series of clinical isolates of drug-resistant (DR) Acinetobacter baumannii with diverse drug susceptibility was detected from eight patients in the emergency intensive care unit of Tokai University Hospital. The initial isolate was obtained in March 2010 (A. baumannii Tokai strain 1); subsequently, seven isolates were obtained from patients (A. baumannii Tokai strains 2–8) and one isolate was obtained from an air-fluidized bed used by five of the patients during the 3 months from August to November 2011. The isolates were classified into three types of antimicrobial drug resistance patterns (RRR, SRR and SSR) according to their susceptibility (S) or resistance (R) to imipenem, amikacin and ciprofloxacin, respectively. Genotyping of these isolates by multilocus sequence typing revealed one sequence type, ST208, whilst that by a DiversiLab analysis revealed two subtypes. All the isolates were positive for blaOXA-51-like and blaOXA-66, as assessed by PCR and DNA sequencing. A. baumannii Tokai strains 1–8 and 10 (RRR, SRR and SSR) had quinolone resistance-associated mutations in gyrA/parC, as revealed by DNA sequencing. The ISAba1 upstream of blaOXA-51-like and aminoglycoside resistance-associated gene, armA, were detected in A. baumannii Tokai strains 1–7 and 10 (RRR and SRR) as assessed by PCR. Among the genes encoding resistance–nodulation–division family pumps (adeB, adeG and adeJ) and outer-membrane porins (oprD and carO), overexpression of adeB and adeJ and suppression of oprD and carO were seen in isolates of A. baumannii Tokai strain 2 (RRR), as assessed by real-time PCR. Thus, the molecular characterization of a series of isolates of DR A. baumannii revealed the outbreak of ST208 and diverse antimicrobial drug susceptibilities, which almost correlated with differential gene alterations responsible for each type of drug resistance. Society for General Microbiology 2014-11 /pmc/articles/PMC4209737/ /pubmed/25142965 http://dx.doi.org/10.1099/jmm.0.077503-0 Text en © 2014 The Authors http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Asai, Satomi Umezawa, Kazuo Iwashita, Hideo Ohshima, Toshio Ohashi, Maya Sasaki, Mika Hayashi, Hideki Matsui, Mari Shibayama, Keigo Inokuchi, Sadaki Miyachi, Hayato
spellingShingle	Asai, Satomi Umezawa, Kazuo Iwashita, Hideo Ohshima, Toshio Ohashi, Maya Sasaki, Mika Hayashi, Hideki Matsui, Mari Shibayama, Keigo Inokuchi, Sadaki Miyachi, Hayato An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
author_facet	Asai, Satomi Umezawa, Kazuo Iwashita, Hideo Ohshima, Toshio Ohashi, Maya Sasaki, Mika Hayashi, Hideki Matsui, Mari Shibayama, Keigo Inokuchi, Sadaki Miyachi, Hayato
author_sort	Asai, Satomi
title	An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
title_short	An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
title_full	An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
title_fullStr	An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
title_full_unstemmed	An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit
title_sort	outbreak of blaoxa-51-like- and blaoxa-66-positive acinetobacter baumannii st208 in the emergency intensive care unit
description	A series of clinical isolates of drug-resistant (DR) Acinetobacter baumannii with diverse drug susceptibility was detected from eight patients in the emergency intensive care unit of Tokai University Hospital. The initial isolate was obtained in March 2010 (A. baumannii Tokai strain 1); subsequently, seven isolates were obtained from patients (A. baumannii Tokai strains 2–8) and one isolate was obtained from an air-fluidized bed used by five of the patients during the 3 months from August to November 2011. The isolates were classified into three types of antimicrobial drug resistance patterns (RRR, SRR and SSR) according to their susceptibility (S) or resistance (R) to imipenem, amikacin and ciprofloxacin, respectively. Genotyping of these isolates by multilocus sequence typing revealed one sequence type, ST208, whilst that by a DiversiLab analysis revealed two subtypes. All the isolates were positive for blaOXA-51-like and blaOXA-66, as assessed by PCR and DNA sequencing. A. baumannii Tokai strains 1–8 and 10 (RRR, SRR and SSR) had quinolone resistance-associated mutations in gyrA/parC, as revealed by DNA sequencing. The ISAba1 upstream of blaOXA-51-like and aminoglycoside resistance-associated gene, armA, were detected in A. baumannii Tokai strains 1–7 and 10 (RRR and SRR) as assessed by PCR. Among the genes encoding resistance–nodulation–division family pumps (adeB, adeG and adeJ) and outer-membrane porins (oprD and carO), overexpression of adeB and adeJ and suppression of oprD and carO were seen in isolates of A. baumannii Tokai strain 2 (RRR), as assessed by real-time PCR. Thus, the molecular characterization of a series of isolates of DR A. baumannii revealed the outbreak of ST208 and diverse antimicrobial drug susceptibilities, which almost correlated with differential gene alterations responsible for each type of drug resistance.
publisher	Society for General Microbiology
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209737/
_version_	1613148844504121344

An outbreak of blaOXA-51-like- and blaOXA-66-positive Acinetobacter baumannii ST208 in the emergency intensive care unit

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