Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8+ T cells with detection by ELISPOT and HLA-multimer staining

Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8+ T cells with detection by ELISPOT and HLA-multimer staining

Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-centre panel organised by the Association for Cancer I...

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Bibliographic Details
Main Authors: Chudley, Lindsey, McCann, Katy J., Coleman, Adam, Cazaly, Angelica M., Bidmon, Nicole, Britten, Cedrik M., van der Burg, Sjoerd H., Gouttefangeas, Cecile, Jandus, Camilla, Laske, Karoline, Maurer, Dominik, Romero, Pedro, Schröder, Helene, Stynenbosch, Linda F. M., Walter, Steffen, Welters, Marij J. P., Ottensmeier, Christian H.
Format: Online
Language:English
Published: Springer Berlin Heidelberg 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209099/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209099/

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