Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells

Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells

Plant sterols have shown potent anti-proliferative effects and apoptosis induction against breast and prostate cancers. However, the effect of sterols against hepatic cancer has not been investigated. In the present study, we assessed whether the stigmasterol isolated from Navicula incerta possesses...

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Main Authors: Kim, Young-Sang, Li, Xi-Feng, Kang, Kyong-Hwa, Ryu, BoMi, Kim, Se Kwon
Format: Online
Language:English
Published: Korean Society for Biochemistry and Molecular Biology 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206714/
id pubmed-4206714
recordtype oai_dc
spelling pubmed-42067142014-10-23 Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells Kim, Young-Sang Li, Xi-Feng Kang, Kyong-Hwa Ryu, BoMi Kim, Se Kwon Research Articles Plant sterols have shown potent anti-proliferative effects and apoptosis induction against breast and prostate cancers. However, the effect of sterols against hepatic cancer has not been investigated. In the present study, we assessed whether the stigmasterol isolated from Navicula incerta possesses apoptosis inductive effect in hepatocarcimona (HepG2) cells. According to the results, Stigmasterol has up-regulated the expression of pro-apoptotic gene expressions (Bax, p53) while down-regulating the anti-apoptotic genes (Bcl-2). Probably via mitochondrial apoptosis signaling pathway. With the induction of apoptosis caspase-8, 9 were activated. The DNA damage and increase in apoptotic cell numbers were observed through Hoechst staining, annexin V staining and cell cycle analysis. According to these results, we can suggest that the stigmasterol shows potent apoptosis inductive effects and has the potential to be tested as an anti-cancer therapeutic against liver cancer. [BMB Reports 2014; 47(8): 433-438] Korean Society for Biochemistry and Molecular Biology 2014-08 /pmc/articles/PMC4206714/ /pubmed/24286323 http://dx.doi.org/10.5483/BMBRep.2014.47.8.153 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Kim, Young-Sang
Li, Xi-Feng
Kang, Kyong-Hwa
Ryu, BoMi
Kim, Se Kwon
spellingShingle Kim, Young-Sang
Li, Xi-Feng
Kang, Kyong-Hwa
Ryu, BoMi
Kim, Se Kwon
Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
author_facet Kim, Young-Sang
Li, Xi-Feng
Kang, Kyong-Hwa
Ryu, BoMi
Kim, Se Kwon
author_sort Kim, Young-Sang
title Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
title_short Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
title_full Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
title_fullStr Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
title_full_unstemmed Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells
title_sort stigmasterol isolated from marine microalgae navicula incerta induces apoptosis in human hepatoma hepg2 cells
description Plant sterols have shown potent anti-proliferative effects and apoptosis induction against breast and prostate cancers. However, the effect of sterols against hepatic cancer has not been investigated. In the present study, we assessed whether the stigmasterol isolated from Navicula incerta possesses apoptosis inductive effect in hepatocarcimona (HepG2) cells. According to the results, Stigmasterol has up-regulated the expression of pro-apoptotic gene expressions (Bax, p53) while down-regulating the anti-apoptotic genes (Bcl-2). Probably via mitochondrial apoptosis signaling pathway. With the induction of apoptosis caspase-8, 9 were activated. The DNA damage and increase in apoptotic cell numbers were observed through Hoechst staining, annexin V staining and cell cycle analysis. According to these results, we can suggest that the stigmasterol shows potent apoptosis inductive effects and has the potential to be tested as an anti-cancer therapeutic against liver cancer. [BMB Reports 2014; 47(8): 433-438]
publisher Korean Society for Biochemistry and Molecular Biology
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206714/
_version_ 1613147816420442112

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