Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil

A Mucoralean fungus was isolated from Caatinga soil of Pernambuco, Northeast of Brazil, and was identified as Cunninghamella echinulata by morphological, physiological, and biochemical tests. This strain was evaluated for biosurfactant/bioemulsifier production using soybean oil waste (SOW) and corn...

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Main Authors:	Silva, Nadielly R. Andrade, Luna, Marcos A. C., Santiago, André L. C. M. A., Franco, Luciana O., Silva, Grayce K. B., de Souza, Patrícia M., Okada, Kaoru, Albuquerque, Clarissa D. C., da Silva, Carlos A. Alves, Campos-Takaki, Galba M.
Format:	Online
Language:	English
Published:	MDPI 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200836/

id	pubmed-4200836
recordtype	oai_dc
spelling	pubmed-42008362014-10-17 Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil Silva, Nadielly R. Andrade Luna, Marcos A. C. Santiago, André L. C. M. A. Franco, Luciana O. Silva, Grayce K. B. de Souza, Patrícia M. Okada, Kaoru Albuquerque, Clarissa D. C. da Silva, Carlos A. Alves Campos-Takaki, Galba M. Article A Mucoralean fungus was isolated from Caatinga soil of Pernambuco, Northeast of Brazil, and was identified as Cunninghamella echinulata by morphological, physiological, and biochemical tests. This strain was evaluated for biosurfactant/bioemulsifier production using soybean oil waste (SOW) and corn steep liquor (CSL) as substrates, added to basic saline solution, by measuring surface tension and emulsifier index and activity. The best results showed the surface water tension was reduced from 72 to 36 mN/m, and an emulsification index (E24) of 80% was obtained using engine oil and burnt engine oil, respectively. A new molecule of biosurfactant showed an anionic charge and a polymeric chemical composition consisting of lipids (40.0% w/w), carbohydrates (35.2% w/w) and protein (20.3% w/w). In addition, the biosurfactant solution (1%) demonstrated its ability for an oil displacement area (ODA) of 37.36 cm2, which is quite similar to that for Triton X-100 (38.46 cm2). The stability of the reduction in the surface water tension as well as of the emulsifier index proved to be stable over a wide range of temperatures, in pH, and in salt concentration (4%–6% w/v). The biosurfactant showed an ability to reduce and increase the viscosity of hydrophobic substrates and their molecules, suggesting that it is a suitable candidate for mediated enhanced oil recovery. At the same time, these studies indicate that renewable, relatively inexpensive and easily available resources can be used for important biotechnological processes. MDPI 2014-09-01 /pmc/articles/PMC4200836/ /pubmed/25257520 http://dx.doi.org/10.3390/ijms150915377 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Silva, Nadielly R. Andrade Luna, Marcos A. C. Santiago, André L. C. M. A. Franco, Luciana O. Silva, Grayce K. B. de Souza, Patrícia M. Okada, Kaoru Albuquerque, Clarissa D. C. da Silva, Carlos A. Alves Campos-Takaki, Galba M.
spellingShingle	Silva, Nadielly R. Andrade Luna, Marcos A. C. Santiago, André L. C. M. A. Franco, Luciana O. Silva, Grayce K. B. de Souza, Patrícia M. Okada, Kaoru Albuquerque, Clarissa D. C. da Silva, Carlos A. Alves Campos-Takaki, Galba M. Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
author_facet	Silva, Nadielly R. Andrade Luna, Marcos A. C. Santiago, André L. C. M. A. Franco, Luciana O. Silva, Grayce K. B. de Souza, Patrícia M. Okada, Kaoru Albuquerque, Clarissa D. C. da Silva, Carlos A. Alves Campos-Takaki, Galba M.
author_sort	Silva, Nadielly R. Andrade
title	Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
title_short	Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
title_full	Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
title_fullStr	Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
title_full_unstemmed	Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil
title_sort	biosurfactant-and-bioemulsifier produced by a promising cunninghamella echinulata isolated from caatinga soil in the northeast of brazil
description	A Mucoralean fungus was isolated from Caatinga soil of Pernambuco, Northeast of Brazil, and was identified as Cunninghamella echinulata by morphological, physiological, and biochemical tests. This strain was evaluated for biosurfactant/bioemulsifier production using soybean oil waste (SOW) and corn steep liquor (CSL) as substrates, added to basic saline solution, by measuring surface tension and emulsifier index and activity. The best results showed the surface water tension was reduced from 72 to 36 mN/m, and an emulsification index (E24) of 80% was obtained using engine oil and burnt engine oil, respectively. A new molecule of biosurfactant showed an anionic charge and a polymeric chemical composition consisting of lipids (40.0% w/w), carbohydrates (35.2% w/w) and protein (20.3% w/w). In addition, the biosurfactant solution (1%) demonstrated its ability for an oil displacement area (ODA) of 37.36 cm2, which is quite similar to that for Triton X-100 (38.46 cm2). The stability of the reduction in the surface water tension as well as of the emulsifier index proved to be stable over a wide range of temperatures, in pH, and in salt concentration (4%–6% w/v). The biosurfactant showed an ability to reduce and increase the viscosity of hydrophobic substrates and their molecules, suggesting that it is a suitable candidate for mediated enhanced oil recovery. At the same time, these studies indicate that renewable, relatively inexpensive and easily available resources can be used for important biotechnological processes.
publisher	MDPI
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200836/
_version_	1613146064807788544

Biosurfactant-and-Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil

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