A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability

3D hierarchical layer double hydroxides (LDHs) have attracted extensive interest due to their unique electronic and catalytic properties. Unfortunately, the existing preparation methods require high temperature or toxic organic compounds, which limits the applications of the 3D hierarchical LDHs in...

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Main Authors:	Wang, Min, Bao, Wen-Jing, Wang, Jiong, Wang, Kang, Xu, Jing-Juan, Chen, Hong-Yuan, Xia, Xing-Hua
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194442/

id	pubmed-4194442
recordtype	oai_dc
spelling	pubmed-41944422014-10-21 A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability Wang, Min Bao, Wen-Jing Wang, Jiong Wang, Kang Xu, Jing-Juan Chen, Hong-Yuan Xia, Xing-Hua Article 3D hierarchical layer double hydroxides (LDHs) have attracted extensive interest due to their unique electronic and catalytic properties. Unfortunately, the existing preparation methods require high temperature or toxic organic compounds, which limits the applications of the 3D hierarchical LDHs in biocatalysis and biomedicine. Herein, we present a green strategy to synthesize “Desert Rose Stone”-like Mg-Al-CO3 LDH nanoflowers in situ deposited on aluminum substrates via a coprecipitation method using atmospheric carbon dioxide. Using this method, we construct a novel “Desert Rose Stone”-like nanobiocatalytic system by using HRP as the model enzyme. Compared with the free HRP, the HRP/Mg-Al-LDH nanobiocatalytic system exhibits higher catalytic activity and stability. A smaller apparent Michaelis-Menten constant (0.16 mM) of this system suggests that the encapsulated HRP shows higher affinity towards H2O2. Nature Publishing Group 2014-10-13 /pmc/articles/PMC4194442/ /pubmed/25308506 http://dx.doi.org/10.1038/srep06606 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Min Bao, Wen-Jing Wang, Jiong Wang, Kang Xu, Jing-Juan Chen, Hong-Yuan Xia, Xing-Hua
spellingShingle	Wang, Min Bao, Wen-Jing Wang, Jiong Wang, Kang Xu, Jing-Juan Chen, Hong-Yuan Xia, Xing-Hua A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
author_facet	Wang, Min Bao, Wen-Jing Wang, Jiong Wang, Kang Xu, Jing-Juan Chen, Hong-Yuan Xia, Xing-Hua
author_sort	Wang, Min
title	A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
title_short	A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
title_full	A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
title_fullStr	A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
title_full_unstemmed	A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
title_sort	green approach to the synthesis of novel “desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability
description	3D hierarchical layer double hydroxides (LDHs) have attracted extensive interest due to their unique electronic and catalytic properties. Unfortunately, the existing preparation methods require high temperature or toxic organic compounds, which limits the applications of the 3D hierarchical LDHs in biocatalysis and biomedicine. Herein, we present a green strategy to synthesize “Desert Rose Stone”-like Mg-Al-CO3 LDH nanoflowers in situ deposited on aluminum substrates via a coprecipitation method using atmospheric carbon dioxide. Using this method, we construct a novel “Desert Rose Stone”-like nanobiocatalytic system by using HRP as the model enzyme. Compared with the free HRP, the HRP/Mg-Al-LDH nanobiocatalytic system exhibits higher catalytic activity and stability. A smaller apparent Michaelis-Menten constant (0.16 mM) of this system suggests that the encapsulated HRP shows higher affinity towards H2O2.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194442/
_version_	1613143699121766400

A green approach to the synthesis of novel “Desert rose stone”-like nanobiocatalytic system with excellent enzyme activity and stability

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