Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation
The dynamics and energy conversion of bacteria are strongly associated with bacterial activities, such as survival, spreading of bacterial diseases and their pathogenesis. Although different discoveries have been reported on trapped bacteria (i.e. immobilized bacteria), the investigation on the dyna...
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| Format: | Online |
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Nature Publishing Group
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192617/ |
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pubmed-4192617 |
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pubmed-41926172014-10-21 Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation Xin, Hongbao Liu, Qingyuan Li, Baojun Article The dynamics and energy conversion of bacteria are strongly associated with bacterial activities, such as survival, spreading of bacterial diseases and their pathogenesis. Although different discoveries have been reported on trapped bacteria (i.e. immobilized bacteria), the investigation on the dynamics and energy conversion of motile bacteria in the process of trapping is highly desirable. Here, we report a non-contact optical trapping of motile bacteria using a modified tapered optical fiber. Using Escherichia coli as an example, both single and multiple motile bacteria have been trapped and manipulated in a non-contact manner. Bacterial dynamics has been observed and bacterial energy has been estimated in the trapping process. This non-contact optical trapping provides a new opportunity for better understanding the bacterial dynamics and energy conversion at the single cell level. Nature Publishing Group 2014-10-10 /pmc/articles/PMC4192617/ /pubmed/25300713 http://dx.doi.org/10.1038/srep06576 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Xin, Hongbao Liu, Qingyuan Li, Baojun |
| spellingShingle |
Xin, Hongbao Liu, Qingyuan Li, Baojun Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| author_facet |
Xin, Hongbao Liu, Qingyuan Li, Baojun |
| author_sort |
Xin, Hongbao |
| title |
Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| title_short |
Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| title_full |
Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| title_fullStr |
Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| title_full_unstemmed |
Non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| title_sort |
non-contact fiber-optical trapping of motile bacteria: dynamics observation and energy estimation |
| description |
The dynamics and energy conversion of bacteria are strongly associated with bacterial activities, such as survival, spreading of bacterial diseases and their pathogenesis. Although different discoveries have been reported on trapped bacteria (i.e. immobilized bacteria), the investigation on the dynamics and energy conversion of motile bacteria in the process of trapping is highly desirable. Here, we report a non-contact optical trapping of motile bacteria using a modified tapered optical fiber. Using Escherichia coli as an example, both single and multiple motile bacteria have been trapped and manipulated in a non-contact manner. Bacterial dynamics has been observed and bacterial energy has been estimated in the trapping process. This non-contact optical trapping provides a new opportunity for better understanding the bacterial dynamics and energy conversion at the single cell level. |
| publisher |
Nature Publishing Group |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192617/ |
| _version_ |
1613142980384784384 |