Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire

Expression of the mouse miR-290–295 cluster and its miR-371–373 homolog in human is restricted to early embryos, primordial germ cells, the germ line stem cell compartment of the adult testis and to stem cell lines derived from the early embryonic lineages. Sequencing data suggest considerable seed...

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Main Authors:	Wu, Shuang, Aksoy, Munevver, Shi, Jianting, Houbaviy, Hristo Botev
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182485/

id	pubmed-4182485
recordtype	oai_dc
spelling	pubmed-41824852014-10-07 Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire Wu, Shuang Aksoy, Munevver Shi, Jianting Houbaviy, Hristo Botev Research Article Expression of the mouse miR-290–295 cluster and its miR-371–373 homolog in human is restricted to early embryos, primordial germ cells, the germ line stem cell compartment of the adult testis and to stem cell lines derived from the early embryonic lineages. Sequencing data suggest considerable seed diversification between the seven homologous pre-miRNAs of miR-290–295 but it is not clear if all of the implied miR-290–295 seeds are also conserved in the human miR-371–373 cluster, which consists of only three homologous pre-miRNAs. By employing miRNA target reporters we show that most, if not all, seeds in miR-290–295 are represented in miR-371–373. In the mouse, pre-miR-290, pre-miR-292 and pre-miR-293 express subsets of the miRNA isoforms processed from the single human pre-miR-371. Comparison of the possible miR-290–295/miR-371–373 seed repertoires in placental mammals suggests a model for the evolution of this miRNA cluster family, which would be otherwise difficult to deduce based solely on pre-miRNA sequence comparisons. The conservation of co-expressed seeds that is characteristic of miR-290–295/miR-371–373 should be taken into account in models of the corresponding miRNA-target interaction networks. Public Library of Science 2014-09-30 /pmc/articles/PMC4182485/ /pubmed/25268927 http://dx.doi.org/10.1371/journal.pone.0108519 Text en © 2014 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wu, Shuang Aksoy, Munevver Shi, Jianting Houbaviy, Hristo Botev
spellingShingle	Wu, Shuang Aksoy, Munevver Shi, Jianting Houbaviy, Hristo Botev Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
author_facet	Wu, Shuang Aksoy, Munevver Shi, Jianting Houbaviy, Hristo Botev
author_sort	Wu, Shuang
title	Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
title_short	Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
title_full	Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
title_fullStr	Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
title_full_unstemmed	Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire
title_sort	evolution of the mir-290–295/mir-371–373 cluster family seed repertoire
description	Expression of the mouse miR-290–295 cluster and its miR-371–373 homolog in human is restricted to early embryos, primordial germ cells, the germ line stem cell compartment of the adult testis and to stem cell lines derived from the early embryonic lineages. Sequencing data suggest considerable seed diversification between the seven homologous pre-miRNAs of miR-290–295 but it is not clear if all of the implied miR-290–295 seeds are also conserved in the human miR-371–373 cluster, which consists of only three homologous pre-miRNAs. By employing miRNA target reporters we show that most, if not all, seeds in miR-290–295 are represented in miR-371–373. In the mouse, pre-miR-290, pre-miR-292 and pre-miR-293 express subsets of the miRNA isoforms processed from the single human pre-miR-371. Comparison of the possible miR-290–295/miR-371–373 seed repertoires in placental mammals suggests a model for the evolution of this miRNA cluster family, which would be otherwise difficult to deduce based solely on pre-miRNA sequence comparisons. The conservation of co-expressed seeds that is characteristic of miR-290–295/miR-371–373 should be taken into account in models of the corresponding miRNA-target interaction networks.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182485/
_version_	1613139918543912960

Evolution of the miR-290–295/miR-371–373 Cluster Family Seed Repertoire

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