Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni

Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni

Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and...

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Main Authors: Nishiyama, Keita, Seto, Yasuyuki, Yoshioka, Kazuki, Kakuda, Tsutomu, Takai, Shinji, Yamamoto, Yuji, Mukai, Takao
Format: Online
Language:English
Published: Public Library of Science 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181664/
id pubmed-4181664
recordtype oai_dc
spelling pubmed-41816642014-10-07 Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni Nishiyama, Keita Seto, Yasuyuki Yoshioka, Kazuki Kakuda, Tsutomu Takai, Shinji Yamamoto, Yuji Mukai, Takao Research Article Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and invasion of Campylobacter jejuni in vitro and to suppress C. jejuni colonization of chicks in vivo. Pretreatment with LG2055 significantly reduced adhesion to and invasion of a human epithelial cell line, Intestine 407, by C. jejuni 81–176. Methanol (MeOH)-fixed LG2055 also reduced infection by C. jejuni 81–176. However, proteinase K (ProK)-treated LG2055 eliminated the inhibitory effects. Moreover, LG2055 co-aggregated with C. jejuni 81–176. ProK treatment prevented this co-aggregation, indicating that the co-aggregation phenotype mediated by the proteinaceous cell-surface components of LG2055 is important for reducing C. jejuni 81–176 adhesion and invasion. In an in vivo assay, oral doses of LG2055 were administered to chicks daily for 14 days after oral inoculation with C. jejuni 81–176. At 14 days post-inoculation, chicks treated with LG2055 had significantly reduced cecum colonization by C. jejuni. Reduction in the number of C. jejuni 81–176 cells adhering to and internalized by human epithelial cells demonstrated that LG2055 is an organism that effectively and competitively excludes C. jejuni 81–176. In addition, the results of the chick colonization assay suggest that treatment with LG2055 could be useful in suppressing C. jejuni colonization of the chicks at early growth stages. Public Library of Science 2014-09-29 /pmc/articles/PMC4181664/ /pubmed/25264604 http://dx.doi.org/10.1371/journal.pone.0108827 Text en © 2014 Nishiyama et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Nishiyama, Keita
Seto, Yasuyuki
Yoshioka, Kazuki
Kakuda, Tsutomu
Takai, Shinji
Yamamoto, Yuji
Mukai, Takao
spellingShingle Nishiyama, Keita
Seto, Yasuyuki
Yoshioka, Kazuki
Kakuda, Tsutomu
Takai, Shinji
Yamamoto, Yuji
Mukai, Takao
Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
author_facet Nishiyama, Keita
Seto, Yasuyuki
Yoshioka, Kazuki
Kakuda, Tsutomu
Takai, Shinji
Yamamoto, Yuji
Mukai, Takao
author_sort Nishiyama, Keita
title Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
title_short Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
title_full Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
title_fullStr Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
title_full_unstemmed Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni
title_sort lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni
description Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and invasion of Campylobacter jejuni in vitro and to suppress C. jejuni colonization of chicks in vivo. Pretreatment with LG2055 significantly reduced adhesion to and invasion of a human epithelial cell line, Intestine 407, by C. jejuni 81–176. Methanol (MeOH)-fixed LG2055 also reduced infection by C. jejuni 81–176. However, proteinase K (ProK)-treated LG2055 eliminated the inhibitory effects. Moreover, LG2055 co-aggregated with C. jejuni 81–176. ProK treatment prevented this co-aggregation, indicating that the co-aggregation phenotype mediated by the proteinaceous cell-surface components of LG2055 is important for reducing C. jejuni 81–176 adhesion and invasion. In an in vivo assay, oral doses of LG2055 were administered to chicks daily for 14 days after oral inoculation with C. jejuni 81–176. At 14 days post-inoculation, chicks treated with LG2055 had significantly reduced cecum colonization by C. jejuni. Reduction in the number of C. jejuni 81–176 cells adhering to and internalized by human epithelial cells demonstrated that LG2055 is an organism that effectively and competitively excludes C. jejuni 81–176. In addition, the results of the chick colonization assay suggest that treatment with LG2055 could be useful in suppressing C. jejuni colonization of the chicks at early growth stages.
publisher Public Library of Science
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4181664/
_version_ 1613139591536050176

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