Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion

The replication of picornaviruses has been described to cause fragmentation of the Golgi apparatus that blocks the secretory pathway. The inhibition of major histocompatibility complex class I upregulation and cytokine, chemokine and interferon secretion may have important implications for host defe...

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Main Authors:	Mousnier, Aurelie, Swieboda, Dawid, Pinto, Anaïs, Guedán, Anabel, Rogers, Andrew V., Walton, Ross, Johnston, Sebastian L., Solari, Roberto
Format:	Online
Language:	English
Published:	American Society for Microbiology 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178721/

id	pubmed-4178721
recordtype	oai_dc
spelling	pubmed-41787212015-04-01 Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion Mousnier, Aurelie Swieboda, Dawid Pinto, Anaïs Guedán, Anabel Rogers, Andrew V. Walton, Ross Johnston, Sebastian L. Solari, Roberto Virus-Cell Interactions The replication of picornaviruses has been described to cause fragmentation of the Golgi apparatus that blocks the secretory pathway. The inhibition of major histocompatibility complex class I upregulation and cytokine, chemokine and interferon secretion may have important implications for host defense. Previous studies have shown that disruption of the secretory pathway can be replicated by expression of individual nonstructural proteins; however the situation with different serotypes of human rhinovirus (HRV) is unclear. The expression of 3A protein from HRV14 or HRV2 did not cause Golgi apparatus disruption or a block in secretion, whereas other studies showed that infection of cells with HRV1A did cause Golgi apparatus disruption which was replicated by the expression of 3A. HRV16 is the serotype most widely used in clinical HRV challenge studies; consequently, to address the issue of Golgi apparatus disruption for HRV16, we have systematically and quantitatively examined the effect of HRV16 on both Golgi apparatus fragmentation and protein secretion in HeLa cells. First, we expressed each individual nonstructural protein and examined their cellular localization and their disruption of endoplasmic reticulum and Golgi apparatus architecture. We quantified their effects on the secretory pathway by measuring secretion of the reporter protein Gaussia luciferase. Finally, we examined the same outcomes following infection of cells with live virus. We demonstrate that expression of HRV16 3A and 3AB and, to a lesser extent, 2B caused dispersal of the Golgi structure, and these three nonstructural proteins also inhibited protein secretion. The infection of cells with HRV16 also caused significant Golgi apparatus dispersal; however, this did not result in the inhibition of protein secretion. American Society for Microbiology 2014-10 /pmc/articles/PMC4178721/ /pubmed/25100828 http://dx.doi.org/10.1128/JVI.01170-14 Text en Copyright © 2014 Mousnier et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mousnier, Aurelie Swieboda, Dawid Pinto, Anaïs Guedán, Anabel Rogers, Andrew V. Walton, Ross Johnston, Sebastian L. Solari, Roberto
spellingShingle	Mousnier, Aurelie Swieboda, Dawid Pinto, Anaïs Guedán, Anabel Rogers, Andrew V. Walton, Ross Johnston, Sebastian L. Solari, Roberto Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
author_facet	Mousnier, Aurelie Swieboda, Dawid Pinto, Anaïs Guedán, Anabel Rogers, Andrew V. Walton, Ross Johnston, Sebastian L. Solari, Roberto
author_sort	Mousnier, Aurelie
title	Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
title_short	Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
title_full	Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
title_fullStr	Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
title_full_unstemmed	Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion
title_sort	human rhinovirus 16 causes golgi apparatus fragmentation without blocking protein secretion
description	The replication of picornaviruses has been described to cause fragmentation of the Golgi apparatus that blocks the secretory pathway. The inhibition of major histocompatibility complex class I upregulation and cytokine, chemokine and interferon secretion may have important implications for host defense. Previous studies have shown that disruption of the secretory pathway can be replicated by expression of individual nonstructural proteins; however the situation with different serotypes of human rhinovirus (HRV) is unclear. The expression of 3A protein from HRV14 or HRV2 did not cause Golgi apparatus disruption or a block in secretion, whereas other studies showed that infection of cells with HRV1A did cause Golgi apparatus disruption which was replicated by the expression of 3A. HRV16 is the serotype most widely used in clinical HRV challenge studies; consequently, to address the issue of Golgi apparatus disruption for HRV16, we have systematically and quantitatively examined the effect of HRV16 on both Golgi apparatus fragmentation and protein secretion in HeLa cells. First, we expressed each individual nonstructural protein and examined their cellular localization and their disruption of endoplasmic reticulum and Golgi apparatus architecture. We quantified their effects on the secretory pathway by measuring secretion of the reporter protein Gaussia luciferase. Finally, we examined the same outcomes following infection of cells with live virus. We demonstrate that expression of HRV16 3A and 3AB and, to a lesser extent, 2B caused dispersal of the Golgi structure, and these three nonstructural proteins also inhibited protein secretion. The infection of cells with HRV16 also caused significant Golgi apparatus dispersal; however, this did not result in the inhibition of protein secretion.
publisher	American Society for Microbiology
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178721/
_version_	1613138588323545088

Human Rhinovirus 16 Causes Golgi Apparatus Fragmentation without Blocking Protein Secretion

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