Wide-field, high-resolution Fourier ptychographic microscopy
In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront c...
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2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169052/ |
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pubmed-41690522014-09-19 Wide-field, high-resolution Fourier ptychographic microscopy Zheng, Guoan Horstmeyer, Roarke Yang, Changhuei Article In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront correction strategy, the FPM method can also correct for aberrations and digitally extend a microscope’s depth-of-focus beyond the physical limitations of its optics. As a demonstration, we built a microscope prototype with a resolution of 0.78 μm, a field-of-view of ~120 mm2, and a resolution-invariant depth-of-focus of 0.3 mm (characterized at 632 nm). Gigapixel colour images of histology slides verify FPM’s successful operation. The reported imaging procedure transforms the general challenge of high-throughput, high-resolution microscopy from one that is coupled to the physical limitations of the system’s optics to one that is solvable through computation. 2013-07-28 2013-09-01 /pmc/articles/PMC4169052/ /pubmed/25243016 http://dx.doi.org/10.1038/nphoton.2013.187 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Zheng, Guoan Horstmeyer, Roarke Yang, Changhuei |
| spellingShingle |
Zheng, Guoan Horstmeyer, Roarke Yang, Changhuei Wide-field, high-resolution Fourier ptychographic microscopy |
| author_facet |
Zheng, Guoan Horstmeyer, Roarke Yang, Changhuei |
| author_sort |
Zheng, Guoan |
| title |
Wide-field, high-resolution Fourier ptychographic microscopy |
| title_short |
Wide-field, high-resolution Fourier ptychographic microscopy |
| title_full |
Wide-field, high-resolution Fourier ptychographic microscopy |
| title_fullStr |
Wide-field, high-resolution Fourier ptychographic microscopy |
| title_full_unstemmed |
Wide-field, high-resolution Fourier ptychographic microscopy |
| title_sort |
wide-field, high-resolution fourier ptychographic microscopy |
| description |
In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront correction strategy, the FPM method can also correct for aberrations and digitally extend a microscope’s depth-of-focus beyond the physical limitations of its optics. As a demonstration, we built a microscope prototype with a resolution of 0.78 μm, a field-of-view of ~120 mm2, and a resolution-invariant depth-of-focus of 0.3 mm (characterized at 632 nm). Gigapixel colour images of histology slides verify FPM’s successful operation. The reported imaging procedure transforms the general challenge of high-throughput, high-resolution microscopy from one that is coupled to the physical limitations of the system’s optics to one that is solvable through computation. |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169052/ |
| _version_ |
1613135493945360384 |