Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules

Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here th...

Full description

Bibliographic Details
Main Authors:	Yunos, Nina Yusrina Muhamad, Tan, Wen-Si, Koh, Chong-Lek, Sam, Choon-Kook, Mohamad, Nur Izzati, Tan, Pui-Wan, Adrian, Tan-Guan-Sheng, Yin, Wai-Fong, Chan, Kok-Gan
Format:	Online
Language:	English
Published:	MDPI 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168423/

id	pubmed-4168423
recordtype	oai_dc
spelling	pubmed-41684232014-09-19 Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules Yunos, Nina Yusrina Muhamad Tan, Wen-Si Koh, Chong-Lek Sam, Choon-Kook Mohamad, Nur Izzati Tan, Pui-Wan Adrian, Tan-Guan-Sheng Yin, Wai-Fong Chan, Kok-Gan Article Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-l-homoserine lactone (C8-HSL) and N-decanoyl-l-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07. MDPI 2014-06-30 /pmc/articles/PMC4168423/ /pubmed/24984061 http://dx.doi.org/10.3390/s140711595 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yunos, Nina Yusrina Muhamad Tan, Wen-Si Koh, Chong-Lek Sam, Choon-Kook Mohamad, Nur Izzati Tan, Pui-Wan Adrian, Tan-Guan-Sheng Yin, Wai-Fong Chan, Kok-Gan
spellingShingle	Yunos, Nina Yusrina Muhamad Tan, Wen-Si Koh, Chong-Lek Sam, Choon-Kook Mohamad, Nur Izzati Tan, Pui-Wan Adrian, Tan-Guan-Sheng Yin, Wai-Fong Chan, Kok-Gan Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
author_facet	Yunos, Nina Yusrina Muhamad Tan, Wen-Si Koh, Chong-Lek Sam, Choon-Kook Mohamad, Nur Izzati Tan, Pui-Wan Adrian, Tan-Guan-Sheng Yin, Wai-Fong Chan, Kok-Gan
author_sort	Yunos, Nina Yusrina Muhamad
title	Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
title_short	Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
title_full	Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
title_fullStr	Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
title_full_unstemmed	Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules
title_sort	pseudomonas cremoricolorata strain nd07 produces n-acyl homoserine lactones as quorum sensing molecules
description	Quorum sensing (QS) is a bacterial cell-to-cell communication system controlling QS-mediated genes which is synchronized with the population density. The regulation of specific gene activity is dependent on the signaling molecules produced, namely N-acyl homoserine lactones (AHLs). We report here the identification and characterization of AHLs produced by bacterial strain ND07 isolated from a Malaysian fresh water sample. Molecular identification showed that strain ND07 is clustered closely to Pseudomonas cremoricolorata. Spent culture supernatant extract of P. cremoricolorata strain ND07 activated the AHL biosensor Chromobacterium violaceum CV026. Using high resolution triple quadrupole liquid chromatography-mass spectrometry, it was confirmed that P. cremoricolorata strain ND07 produced N-octanoyl-l-homoserine lactone (C8-HSL) and N-decanoyl-l-homoserine lactone (C10-HSL). To the best of our knowledge, this is the first documentation on the production of C10-HSL in P. cremoricolorata strain ND07.
publisher	MDPI
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168423/
_version_	1613135218150998016

Pseudomonas cremoricolorata Strain ND07 Produces N-acyl Homoserine Lactones as Quorum Sensing Molecules

Similar Items