Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage

Blueberry is a plant with a number of nutritional and biomedical capabilities. In the present study we initially evaluated the capacity of its juice (BJ) to inhibit the number of aberrant crypts (AC) induced with azoxymethane (AOM) in mouse. BJ was administered daily by the oral route to three group...

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Main Authors:	Álvarez-González, Isela, Garcia-Melo, Fernando, Vásquez-Garzón, Verónica R., Villa-Treviño, Saúl, Madrigal-Santillán, E. Osiris, Morales-González, José A., Mendoza-Pérez, Jorge A., Madrigal-Bujaidar, Eduardo
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166644/

id	pubmed-4166644
recordtype	oai_dc
spelling	pubmed-41666442014-09-25 Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage Álvarez-González, Isela Garcia-Melo, Fernando Vásquez-Garzón, Verónica R. Villa-Treviño, Saúl Madrigal-Santillán, E. Osiris Morales-González, José A. Mendoza-Pérez, Jorge A. Madrigal-Bujaidar, Eduardo Research Article Blueberry is a plant with a number of nutritional and biomedical capabilities. In the present study we initially evaluated the capacity of its juice (BJ) to inhibit the number of aberrant crypts (AC) induced with azoxymethane (AOM) in mouse. BJ was administered daily by the oral route to three groups of animals during four weeks (1.6, 4.1, and 15.0 μL/g), respectively, while AOM (10 mg/kg) was intraperitoneally injected to the mentioned groups, twice a week, in weeks two and three of the assay. We also included two control groups of mice, one administered distilled water and the other the high dose of BJ. A significant increase of AC was observed in the AOM treated animals, and a mean protection of 75.6% was determined with the two low doses of BJ tested; however, the high dose of the juice administered together with AOM increased the number of crypts more than four times the value observed in animals administered only AOM. Furthermore, we determined the antioxidant potential of BJ with an ex vivo DPPH assay and found a dose-dependent decrease with a mean of 19.5%. We also determined the DNA oxidation/antioxidation by identifying 8-hydroxy-2′-deoxyguanosine adducts and found a mean decrease of 44.3% with the BJ administration with respect to the level induced by AOM. Our results show a complex differential effect of BJ related to the tested doses, opening the need to further evaluate a number of factors so as to determine the possibility of a cocarcinogenic potential. Hindawi Publishing Corporation 2014 2014-09-03 /pmc/articles/PMC4166644/ /pubmed/25258642 http://dx.doi.org/10.1155/2014/379890 Text en Copyright © 2014 Isela Álvarez-González et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Álvarez-González, Isela Garcia-Melo, Fernando Vásquez-Garzón, Verónica R. Villa-Treviño, Saúl Madrigal-Santillán, E. Osiris Morales-González, José A. Mendoza-Pérez, Jorge A. Madrigal-Bujaidar, Eduardo
spellingShingle	Álvarez-González, Isela Garcia-Melo, Fernando Vásquez-Garzón, Verónica R. Villa-Treviño, Saúl Madrigal-Santillán, E. Osiris Morales-González, José A. Mendoza-Pérez, Jorge A. Madrigal-Bujaidar, Eduardo Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
author_facet	Álvarez-González, Isela Garcia-Melo, Fernando Vásquez-Garzón, Verónica R. Villa-Treviño, Saúl Madrigal-Santillán, E. Osiris Morales-González, José A. Mendoza-Pérez, Jorge A. Madrigal-Bujaidar, Eduardo
author_sort	Álvarez-González, Isela
title	Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
title_short	Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
title_full	Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
title_fullStr	Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
title_full_unstemmed	Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage
title_sort	evaluation of blueberry juice in mouse azoxymethane-induced aberrant crypts and oxidative damage
description	Blueberry is a plant with a number of nutritional and biomedical capabilities. In the present study we initially evaluated the capacity of its juice (BJ) to inhibit the number of aberrant crypts (AC) induced with azoxymethane (AOM) in mouse. BJ was administered daily by the oral route to three groups of animals during four weeks (1.6, 4.1, and 15.0 μL/g), respectively, while AOM (10 mg/kg) was intraperitoneally injected to the mentioned groups, twice a week, in weeks two and three of the assay. We also included two control groups of mice, one administered distilled water and the other the high dose of BJ. A significant increase of AC was observed in the AOM treated animals, and a mean protection of 75.6% was determined with the two low doses of BJ tested; however, the high dose of the juice administered together with AOM increased the number of crypts more than four times the value observed in animals administered only AOM. Furthermore, we determined the antioxidant potential of BJ with an ex vivo DPPH assay and found a dose-dependent decrease with a mean of 19.5%. We also determined the DNA oxidation/antioxidation by identifying 8-hydroxy-2′-deoxyguanosine adducts and found a mean decrease of 44.3% with the BJ administration with respect to the level induced by AOM. Our results show a complex differential effect of BJ related to the tested doses, opening the need to further evaluate a number of factors so as to determine the possibility of a cocarcinogenic potential.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4166644/
_version_	1613134723269263360

Evaluation of Blueberry Juice in Mouse Azoxymethane-Induced Aberrant Crypts and Oxidative Damage

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