siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa
To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were...
Main Authors: | , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Korean Society for Biochemistry and Molecular Biology
2014
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163889/ |