Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *

The colonic epithelium is a highly dynamic system important for the regulation of ion and water homeostasis via absorption and secretion and for the maintenance of a protective barrier between the outer milieu and the inside of the body. These processes are known to gradually change along the length...

Full description

Bibliographic Details
Main Authors:	van der Post, Sjoerd, Hansson, Gunnar C.
Format:	Online
Language:	English
Published:	The American Society for Biochemistry and Molecular Biology 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159649/

id	pubmed-4159649
recordtype	oai_dc
spelling	pubmed-41596492014-09-10 Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences * van der Post, Sjoerd Hansson, Gunnar C. Research The colonic epithelium is a highly dynamic system important for the regulation of ion and water homeostasis via absorption and secretion and for the maintenance of a protective barrier between the outer milieu and the inside of the body. These processes are known to gradually change along the length of the colon, although a complete characterization at the protein level is lacking. We therefore analyzed the membrane proteome of isolated human (n = 4) colonic epithelial cells from biopsies obtained via routine colonoscopy for four segments along the large intestine: ascending, transverse, descending, and sigmoid colon. Label-free quantitative proteomic analyses using high-resolution mass spectrometry were performed on enriched membrane proteins. The results showed a stable level for the majority of membrane proteins but a distinct decrease in proteins associated with bacterial sensing, cation transport, and O-glycosylation in the proximal to distal regions. In contrast, proteins involved in microbial defense and anion transport showed an opposing gradient and increased toward the distal end. The gradient of ion-transporter proteins could be directly related to previously observed ion transport activities. All individual glycosyltransferases required for the O-glycosylation of the major colonic mucin MUC2 were observed and correlated with the known glycosylation variation along the colon axis. This is the first comprehensive quantitative dataset of membrane protein abundance along the human colon and will add to the knowledge of the physiological function of the different regions of the colonic mucosa. Mass spectrometry data have been deposited to the ProteomeXchange with the identifier PXD000987. The American Society for Biochemistry and Molecular Biology 2014-09 2014-06-02 /pmc/articles/PMC4159649/ /pubmed/24889196 http://dx.doi.org/10.1074/mcp.M114.040204 Text en © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	van der Post, Sjoerd Hansson, Gunnar C.
spellingShingle	van der Post, Sjoerd Hansson, Gunnar C. Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
author_facet	van der Post, Sjoerd Hansson, Gunnar C.
author_sort	van der Post, Sjoerd
title	Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
title_short	Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
title_full	Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
title_fullStr	Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
title_full_unstemmed	Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *
title_sort	membrane protein profiling of human colon reveals distinct regional differences *
description	The colonic epithelium is a highly dynamic system important for the regulation of ion and water homeostasis via absorption and secretion and for the maintenance of a protective barrier between the outer milieu and the inside of the body. These processes are known to gradually change along the length of the colon, although a complete characterization at the protein level is lacking. We therefore analyzed the membrane proteome of isolated human (n = 4) colonic epithelial cells from biopsies obtained via routine colonoscopy for four segments along the large intestine: ascending, transverse, descending, and sigmoid colon. Label-free quantitative proteomic analyses using high-resolution mass spectrometry were performed on enriched membrane proteins. The results showed a stable level for the majority of membrane proteins but a distinct decrease in proteins associated with bacterial sensing, cation transport, and O-glycosylation in the proximal to distal regions. In contrast, proteins involved in microbial defense and anion transport showed an opposing gradient and increased toward the distal end. The gradient of ion-transporter proteins could be directly related to previously observed ion transport activities. All individual glycosyltransferases required for the O-glycosylation of the major colonic mucin MUC2 were observed and correlated with the known glycosylation variation along the colon axis. This is the first comprehensive quantitative dataset of membrane protein abundance along the human colon and will add to the knowledge of the physiological function of the different regions of the colonic mucosa. Mass spectrometry data have been deposited to the ProteomeXchange with the identifier PXD000987.
publisher	The American Society for Biochemistry and Molecular Biology
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159649/
_version_	1613132581288542208

Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences *

Similar Items