Differential recombination dynamics within the MHC of macaque species

Differential recombination dynamics within the MHC of macaque species

A panel of 15 carefully selected microsatellites (short tandem repeats, STRs) has allowed us to study segregation and haplotype stability in various macaque species. The STRs span the major histocompatibility complex (MHC) region and map in more detail from the centromeric part of the Mhc-A to the D...

Full description

Bibliographic Details
Main Authors: de Groot, Nanine, Doxiadis, Gaby G. M., Otting, Nel, de Vos-Rouweler, Annemiek J. M., Bontrop, Ronald E.
Format: Online
Language:English
Published: Springer Berlin Heidelberg 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156779/
id pubmed-4156779
recordtype oai_dc
spelling pubmed-41567792014-09-08 Differential recombination dynamics within the MHC of macaque species de Groot, Nanine Doxiadis, Gaby G. M. Otting, Nel de Vos-Rouweler, Annemiek J. M. Bontrop, Ronald E. Original Paper A panel of 15 carefully selected microsatellites (short tandem repeats, STRs) has allowed us to study segregation and haplotype stability in various macaque species. The STRs span the major histocompatibility complex (MHC) region and map in more detail from the centromeric part of the Mhc-A to the DR region. Two large panels of Indian rhesus and Indonesian/Indochinese cynomolgus macaques have been subjected to pedigree analysis, allowing the definition of 161 and 36 different haplotypes and the physical mapping of 10 and 5 recombination sites, respectively. Although most recombination sites within the studied section of the Indian rhesus monkey MHC are situated between the Mhc-A and Mhc-B regions, the resulting recombination rate for this genomic segment is low and similar to that in humans. In contrast, in Indonesian/Indochinese macaques, two recombination sites, which appear to be absent in rhesus macaques, map between the class III and II regions. As a result, the mean recombination frequency of the core MHC, Mhc-A to class II, is higher in Indonesian/Indochinese cynomolgus than in Indian rhesus macaques, but as such is comparable to that in humans. The present communication demonstrates that the dynamics of recombination ‘hot/cold spots’ in the MHC, as well as their frequencies, may differ substantially between highly related macaque species. Springer Berlin Heidelberg 2014-06-17 2014 /pmc/articles/PMC4156779/ /pubmed/24934118 http://dx.doi.org/10.1007/s00251-014-0783-4 Text en © The Author(s) 2014 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author de Groot, Nanine
Doxiadis, Gaby G. M.
Otting, Nel
de Vos-Rouweler, Annemiek J. M.
Bontrop, Ronald E.
spellingShingle de Groot, Nanine
Doxiadis, Gaby G. M.
Otting, Nel
de Vos-Rouweler, Annemiek J. M.
Bontrop, Ronald E.
Differential recombination dynamics within the MHC of macaque species
author_facet de Groot, Nanine
Doxiadis, Gaby G. M.
Otting, Nel
de Vos-Rouweler, Annemiek J. M.
Bontrop, Ronald E.
author_sort de Groot, Nanine
title Differential recombination dynamics within the MHC of macaque species
title_short Differential recombination dynamics within the MHC of macaque species
title_full Differential recombination dynamics within the MHC of macaque species
title_fullStr Differential recombination dynamics within the MHC of macaque species
title_full_unstemmed Differential recombination dynamics within the MHC of macaque species
title_sort differential recombination dynamics within the mhc of macaque species
description A panel of 15 carefully selected microsatellites (short tandem repeats, STRs) has allowed us to study segregation and haplotype stability in various macaque species. The STRs span the major histocompatibility complex (MHC) region and map in more detail from the centromeric part of the Mhc-A to the DR region. Two large panels of Indian rhesus and Indonesian/Indochinese cynomolgus macaques have been subjected to pedigree analysis, allowing the definition of 161 and 36 different haplotypes and the physical mapping of 10 and 5 recombination sites, respectively. Although most recombination sites within the studied section of the Indian rhesus monkey MHC are situated between the Mhc-A and Mhc-B regions, the resulting recombination rate for this genomic segment is low and similar to that in humans. In contrast, in Indonesian/Indochinese macaques, two recombination sites, which appear to be absent in rhesus macaques, map between the class III and II regions. As a result, the mean recombination frequency of the core MHC, Mhc-A to class II, is higher in Indonesian/Indochinese cynomolgus than in Indian rhesus macaques, but as such is comparable to that in humans. The present communication demonstrates that the dynamics of recombination ‘hot/cold spots’ in the MHC, as well as their frequencies, may differ substantially between highly related macaque species.
publisher Springer Berlin Heidelberg
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156779/
_version_ 1613131640371937280

Similar Items