Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation

Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from...

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Main Authors:	Kong, Cin, Tan, Man-Wah, Nathan, Sheila
Format:	Online
Language:	English
Published:	The Company of Biologists 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154301/

id	pubmed-4154301
recordtype	oai_dc
spelling	pubmed-41543012014-09-04 Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation Kong, Cin Tan, Man-Wah Nathan, Sheila Research Article Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. One of the hits obtained from the screen was the aqueous extract of Orthosiphon stamineus leaves (UE-12) that enhanced the survival of infected nematodes without interfering with bacterial growth. In this study, we used a fluorescent transgenic reporter strain and observed that the repressed expression of the lys-7 defense gene in infected nematodes was restored in the presence of UE-12. Analysis of a selected panel of PMK-1 and DAF-16-regulated transcripts and loss-of-function mutants in these pathways indicates that the protective role of UE-12 is mediated via the p38 MAP kinase and insulin-like signaling pathways. Further analysis of a panel of known bioactive compounds of UE-12 proposed eupatorin (C18H16O7) as the possible candidate active molecule contributing to the anti-infective property of UE-12. Taken together, these findings strongly suggest that the O. stamineus leaf extract is a promising anti-infective agent that confers an advantage in survival against S. aureus infection by modulating the immune response of the infected host. The Company of Biologists 2014-06-27 /pmc/articles/PMC4154301/ /pubmed/24972867 http://dx.doi.org/10.1242/bio.20148334 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kong, Cin Tan, Man-Wah Nathan, Sheila
spellingShingle	Kong, Cin Tan, Man-Wah Nathan, Sheila Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
author_facet	Kong, Cin Tan, Man-Wah Nathan, Sheila
author_sort	Kong, Cin
title	Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_short	Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_full	Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_fullStr	Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_full_unstemmed	Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_sort	orthosiphon stamineus protects caenorhabditis elegans against staphylococcus aureus infection through immunomodulation
description	Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. One of the hits obtained from the screen was the aqueous extract of Orthosiphon stamineus leaves (UE-12) that enhanced the survival of infected nematodes without interfering with bacterial growth. In this study, we used a fluorescent transgenic reporter strain and observed that the repressed expression of the lys-7 defense gene in infected nematodes was restored in the presence of UE-12. Analysis of a selected panel of PMK-1 and DAF-16-regulated transcripts and loss-of-function mutants in these pathways indicates that the protective role of UE-12 is mediated via the p38 MAP kinase and insulin-like signaling pathways. Further analysis of a panel of known bioactive compounds of UE-12 proposed eupatorin (C18H16O7) as the possible candidate active molecule contributing to the anti-infective property of UE-12. Taken together, these findings strongly suggest that the O. stamineus leaf extract is a promising anti-infective agent that confers an advantage in survival against S. aureus infection by modulating the immune response of the infected host.
publisher	The Company of Biologists
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154301/
_version_	1613130868218396672

Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation

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