Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species

Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species

To understand molecular mechanisms of perennial grass adaptation to drought stress, genes associated with drought avoidance or tolerance traits were identified and their expression patterns were characterized in C4 hybrid bermudagrass [Cynodon dactylon (L.) Pers.×C. transvaalensis Burtt Davy, cv. Ti...

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Main Authors: Zhou, Peng, An, Yuan, Wang, Zhaolong, Du, Hongmei, Huang, Bingru
Format: Online
Language:English
Published: Public Library of Science 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143173/
id pubmed-4143173
recordtype oai_dc
spelling pubmed-41431732014-08-27 Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species Zhou, Peng An, Yuan Wang, Zhaolong Du, Hongmei Huang, Bingru Research Article To understand molecular mechanisms of perennial grass adaptation to drought stress, genes associated with drought avoidance or tolerance traits were identified and their expression patterns were characterized in C4 hybrid bermudagrass [Cynodon dactylon (L.) Pers.×C. transvaalensis Burtt Davy, cv. Tifway] and common bermudagrass (C. dactylon, cv. C299). Plants of drought-tolerant ‘Tifway’ and drought-sensitive ‘C299’ were exposed to drought for 5 d (mild stress) and 10 d (severe stress) by withholding irrigation in a growth chamber. ‘Tifway’ maintained significantly lower electrolyte leakage and higher relative water content than ‘C299’ at both 5 and 10 d of drought stress. Four cDNA libraries via suppression subtractive hybridization analysis were constructed and identified 277 drought-responsive genes in the two genotypes at 5 and 10 d of drought stress, which were mainly classified into the functional categories of stress defense, metabolism, osmoregulation, membrane system, signal and regulator, structural protein, protein synthesis and degradation, and energy metabolism. Quantitative-PCR analysis confirmed the expression of 36 drought up-regulated genes that were more highly expressed in drought-tolerant ‘Tifway’ than drought-sensitive ‘C299’, including those for drought avoidance traits, such as cuticle wax formation (CER1 and sterol desaturase), for drought tolerance traits, such as dehydration-protective proteins (dehydrins, HVA-22-like protein) and oxidative stress defense (superoxide dismutase, dehydroascorbate reductase, 2-Cys peroxiredoxins), and for stress signaling (EREBP-4 like protein and WRKY transcription factor). The results suggest that the expression of genes for stress signaling, cuticle wax accumulation, antioxidant defense, and dehydration-protective protein accumulation could be critically important for warm-season perennial grass adaptation to long-term drought stress. Public Library of Science 2014-08-25 /pmc/articles/PMC4143173/ /pubmed/25153119 http://dx.doi.org/10.1371/journal.pone.0103611 Text en © 2014 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Zhou, Peng
An, Yuan
Wang, Zhaolong
Du, Hongmei
Huang, Bingru
spellingShingle Zhou, Peng
An, Yuan
Wang, Zhaolong
Du, Hongmei
Huang, Bingru
Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
author_facet Zhou, Peng
An, Yuan
Wang, Zhaolong
Du, Hongmei
Huang, Bingru
author_sort Zhou, Peng
title Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
title_short Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
title_full Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
title_fullStr Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
title_full_unstemmed Characterization of Gene Expression Associated with Drought Avoidance and Tolerance Traits in a Perennial Grass Species
title_sort characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species
description To understand molecular mechanisms of perennial grass adaptation to drought stress, genes associated with drought avoidance or tolerance traits were identified and their expression patterns were characterized in C4 hybrid bermudagrass [Cynodon dactylon (L.) Pers.×C. transvaalensis Burtt Davy, cv. Tifway] and common bermudagrass (C. dactylon, cv. C299). Plants of drought-tolerant ‘Tifway’ and drought-sensitive ‘C299’ were exposed to drought for 5 d (mild stress) and 10 d (severe stress) by withholding irrigation in a growth chamber. ‘Tifway’ maintained significantly lower electrolyte leakage and higher relative water content than ‘C299’ at both 5 and 10 d of drought stress. Four cDNA libraries via suppression subtractive hybridization analysis were constructed and identified 277 drought-responsive genes in the two genotypes at 5 and 10 d of drought stress, which were mainly classified into the functional categories of stress defense, metabolism, osmoregulation, membrane system, signal and regulator, structural protein, protein synthesis and degradation, and energy metabolism. Quantitative-PCR analysis confirmed the expression of 36 drought up-regulated genes that were more highly expressed in drought-tolerant ‘Tifway’ than drought-sensitive ‘C299’, including those for drought avoidance traits, such as cuticle wax formation (CER1 and sterol desaturase), for drought tolerance traits, such as dehydration-protective proteins (dehydrins, HVA-22-like protein) and oxidative stress defense (superoxide dismutase, dehydroascorbate reductase, 2-Cys peroxiredoxins), and for stress signaling (EREBP-4 like protein and WRKY transcription factor). The results suggest that the expression of genes for stress signaling, cuticle wax accumulation, antioxidant defense, and dehydration-protective protein accumulation could be critically important for warm-season perennial grass adaptation to long-term drought stress.
publisher Public Library of Science
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143173/
_version_ 1613127199383093248

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