The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates

Recently, we revealed the importance of follicular helper T cells (TFH) in the pathogenesis of primary Sjögren's syndrome (pSS). In the present study, we focused on the site of the inflammation and determined the composition of lymphocyte infiltration in labial salivary gland (LSG) biopsies wit...

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Main Authors:	Szabo, Krisztina, Papp, Gabor, Dezso, Balazs, Zeher, Margit
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142299/

id	pubmed-4142299
recordtype	oai_dc
spelling	pubmed-41422992014-08-31 The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates Szabo, Krisztina Papp, Gabor Dezso, Balazs Zeher, Margit Research Article Recently, we revealed the importance of follicular helper T cells (TFH) in the pathogenesis of primary Sjögren's syndrome (pSS). In the present study, we focused on the site of the inflammation and determined the composition of lymphocyte infiltration in labial salivary gland (LSG) biopsies with special emphasis on TFH and germinal center B cells. We selected tissue blocks obtained from ten patients at the time of disease onset. Detection of cell specific markers was performed with immunohistochemical and immunofluorescence stainings. We evaluated patients' clinical and laboratory features retrospectively and assessed the relation between disease course and early histopathological findings. LSG biopsies were graded based on the extension and arrangement level of periductal inflammatory cell infiltrates. TFH cell markers (CD84, PD-1, and Bcl-6) occurred predominantly in more organized structures with higher focus scores. The coexpression of CD3 and Bcl-6 markers clearly identified TFH cells close to Bcl-6+ B cells with the typical formation of germinal centers. Systemic features were developed later in the disease course only in patients with highly structured infiltrates and the presence of TFH cells. Our observations suggest that the presence of TFH cells in LSGs at the disease onset may predict a more pronounced clinical course of pSS. Hindawi Publishing Corporation 2014 2014-08-07 /pmc/articles/PMC4142299/ /pubmed/25177110 http://dx.doi.org/10.1155/2014/631787 Text en Copyright © 2014 Krisztina Szabo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Szabo, Krisztina Papp, Gabor Dezso, Balazs Zeher, Margit
spellingShingle	Szabo, Krisztina Papp, Gabor Dezso, Balazs Zeher, Margit The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
author_facet	Szabo, Krisztina Papp, Gabor Dezso, Balazs Zeher, Margit
author_sort	Szabo, Krisztina
title	The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
title_short	The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
title_full	The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
title_fullStr	The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
title_full_unstemmed	The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates
title_sort	histopathology of labial salivary glands in primary sjögren's syndrome: focusing on follicular helper t cells in the inflammatory infiltrates
description	Recently, we revealed the importance of follicular helper T cells (TFH) in the pathogenesis of primary Sjögren's syndrome (pSS). In the present study, we focused on the site of the inflammation and determined the composition of lymphocyte infiltration in labial salivary gland (LSG) biopsies with special emphasis on TFH and germinal center B cells. We selected tissue blocks obtained from ten patients at the time of disease onset. Detection of cell specific markers was performed with immunohistochemical and immunofluorescence stainings. We evaluated patients' clinical and laboratory features retrospectively and assessed the relation between disease course and early histopathological findings. LSG biopsies were graded based on the extension and arrangement level of periductal inflammatory cell infiltrates. TFH cell markers (CD84, PD-1, and Bcl-6) occurred predominantly in more organized structures with higher focus scores. The coexpression of CD3 and Bcl-6 markers clearly identified TFH cells close to Bcl-6+ B cells with the typical formation of germinal centers. Systemic features were developed later in the disease course only in patients with highly structured infiltrates and the presence of TFH cells. Our observations suggest that the presence of TFH cells in LSGs at the disease onset may predict a more pronounced clinical course of pSS.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142299/
_version_	1613126950465830912

The Histopathology of Labial Salivary Glands in Primary Sjögren's Syndrome: Focusing on Follicular Helper T Cells in the Inflammatory Infiltrates

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