Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well...

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Main Authors:	Parillo, Francesco, Maranesi, Margherita, Mignini, Fiorenzo, Marinelli, Lisa, Di Stefano, Antonio, Boiti, Cristiano, Zerani, Massimo
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141718/

id	pubmed-4141718
recordtype	oai_dc
spelling	pubmed-41417182014-08-25 Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea Parillo, Francesco Maranesi, Margherita Mignini, Fiorenzo Marinelli, Lisa Di Stefano, Antonio Boiti, Cristiano Zerani, Massimo Research Article Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well as the in vitro effects of DA on hormone productions by CL in pseudopregnant rabbits. Immunoreactivity and gene expression for D1R decreased while that for D3R increased in luteal and blood vessel cells from early to late pseudopregnant stages. DA immunopositivity was evidenced only in luteal cells. The DA and D1R agonist increased in vitro release of progesterone and prostaglandin E2 (PGE2) by early CL, whereas the DA and D3R agonist decreased progesterone and increased PGF2α in vitro release by mid- and late CL. These results provide evidence that the DA/DR system exerts a dual modulatory function in the lifespan of CL: the DA/D1R is luteotropic while the DA/D3R is luteolytic. The present data shed new light on the physiological mechanisms regulating luteal activity that might improve our ability to optimize reproductive efficiency in mammal species, including humans. Public Library of Science 2014-08-22 /pmc/articles/PMC4141718/ /pubmed/25148384 http://dx.doi.org/10.1371/journal.pone.0104797 Text en © 2014 Parillo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Parillo, Francesco Maranesi, Margherita Mignini, Fiorenzo Marinelli, Lisa Di Stefano, Antonio Boiti, Cristiano Zerani, Massimo
spellingShingle	Parillo, Francesco Maranesi, Margherita Mignini, Fiorenzo Marinelli, Lisa Di Stefano, Antonio Boiti, Cristiano Zerani, Massimo Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
author_facet	Parillo, Francesco Maranesi, Margherita Mignini, Fiorenzo Marinelli, Lisa Di Stefano, Antonio Boiti, Cristiano Zerani, Massimo
author_sort	Parillo, Francesco
title	Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_short	Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_full	Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_fullStr	Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_full_unstemmed	Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_sort	evidence for a dopamine intrinsic direct role in the regulation of the ovary reproductive function: in vitro study on rabbit corpora lutea
description	Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well as the in vitro effects of DA on hormone productions by CL in pseudopregnant rabbits. Immunoreactivity and gene expression for D1R decreased while that for D3R increased in luteal and blood vessel cells from early to late pseudopregnant stages. DA immunopositivity was evidenced only in luteal cells. The DA and D1R agonist increased in vitro release of progesterone and prostaglandin E2 (PGE2) by early CL, whereas the DA and D3R agonist decreased progesterone and increased PGF2α in vitro release by mid- and late CL. These results provide evidence that the DA/DR system exerts a dual modulatory function in the lifespan of CL: the DA/D1R is luteotropic while the DA/D3R is luteolytic. The present data shed new light on the physiological mechanisms regulating luteal activity that might improve our ability to optimize reproductive efficiency in mammal species, including humans.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141718/
_version_	1613126766802501632

Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

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