Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest

Permafrost soils are large reservoirs of potentially labile carbon (C). Understanding the dynamics of C release from these soils requires us to account for the impact of wildfires, which are increasing in frequency as the climate changes. Boreal wildfires contribute to global emission of greenhouse...

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Main Authors:	Taş, Neslihan, Prestat, Emmanuel, McFarland, Jack W, Wickland, Kimberley P, Knight, Rob, Berhe, Asmeret Asefaw, Jorgenson, Torre, Waldrop, Mark P, Jansson, Janet K
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139727/

id	pubmed-4139727
recordtype	oai_dc
spelling	pubmed-41397272014-09-01 Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest Taş, Neslihan Prestat, Emmanuel McFarland, Jack W Wickland, Kimberley P Knight, Rob Berhe, Asmeret Asefaw Jorgenson, Torre Waldrop, Mark P Jansson, Janet K Original Article Permafrost soils are large reservoirs of potentially labile carbon (C). Understanding the dynamics of C release from these soils requires us to account for the impact of wildfires, which are increasing in frequency as the climate changes. Boreal wildfires contribute to global emission of greenhouse gases (GHG—CO2, CH4 and N2O) and indirectly result in the thawing of near-surface permafrost. In this study, we aimed to define the impact of fire on soil microbial communities and metabolic potential for GHG fluxes in samples collected up to 1 m depth from an upland black spruce forest near Nome Creek, Alaska. We measured geochemistry, GHG fluxes, potential soil enzyme activities and microbial community structure via 16SrRNA gene and metagenome sequencing. We found that soil moisture, C content and the potential for respiration were reduced by fire, as were microbial community diversity and metabolic potential. There were shifts in dominance of several microbial community members, including a higher abundance of candidate phylum AD3 after fire. The metagenome data showed that fire had a pervasive impact on genes involved in carbohydrate metabolism, methanogenesis and the nitrogen cycle. Although fire resulted in an immediate release of CO2 from surface soils, our results suggest that the potential for emission of GHG was ultimately reduced at all soil depths over the longer term. Because of the size of the permafrost C reservoir, these results are crucial for understanding whether fire produces a positive or negative feedback loop contributing to the global C cycle. Nature Publishing Group 2014-09 2014-04-10 /pmc/articles/PMC4139727/ /pubmed/24722629 http://dx.doi.org/10.1038/ismej.2014.36 Text en Copyright © 2014 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Taş, Neslihan Prestat, Emmanuel McFarland, Jack W Wickland, Kimberley P Knight, Rob Berhe, Asmeret Asefaw Jorgenson, Torre Waldrop, Mark P Jansson, Janet K
spellingShingle	Taş, Neslihan Prestat, Emmanuel McFarland, Jack W Wickland, Kimberley P Knight, Rob Berhe, Asmeret Asefaw Jorgenson, Torre Waldrop, Mark P Jansson, Janet K Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
author_facet	Taş, Neslihan Prestat, Emmanuel McFarland, Jack W Wickland, Kimberley P Knight, Rob Berhe, Asmeret Asefaw Jorgenson, Torre Waldrop, Mark P Jansson, Janet K
author_sort	Taş, Neslihan
title	Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
title_short	Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
title_full	Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
title_fullStr	Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
title_full_unstemmed	Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest
title_sort	impact of fire on active layer and permafrost microbial communities and metagenomes in an upland alaskan boreal forest
description	Permafrost soils are large reservoirs of potentially labile carbon (C). Understanding the dynamics of C release from these soils requires us to account for the impact of wildfires, which are increasing in frequency as the climate changes. Boreal wildfires contribute to global emission of greenhouse gases (GHG—CO2, CH4 and N2O) and indirectly result in the thawing of near-surface permafrost. In this study, we aimed to define the impact of fire on soil microbial communities and metabolic potential for GHG fluxes in samples collected up to 1 m depth from an upland black spruce forest near Nome Creek, Alaska. We measured geochemistry, GHG fluxes, potential soil enzyme activities and microbial community structure via 16SrRNA gene and metagenome sequencing. We found that soil moisture, C content and the potential for respiration were reduced by fire, as were microbial community diversity and metabolic potential. There were shifts in dominance of several microbial community members, including a higher abundance of candidate phylum AD3 after fire. The metagenome data showed that fire had a pervasive impact on genes involved in carbohydrate metabolism, methanogenesis and the nitrogen cycle. Although fire resulted in an immediate release of CO2 from surface soils, our results suggest that the potential for emission of GHG was ultimately reduced at all soil depths over the longer term. Because of the size of the permafrost C reservoir, these results are crucial for understanding whether fire produces a positive or negative feedback loop contributing to the global C cycle.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139727/
_version_	1613126099987857408

Impact of fire on active layer and permafrost microbial communities and metagenomes in an upland Alaskan boreal forest

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