Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro

Since tendon injuries and tendinopathy are a growing problem, sometimes requiring surgery, new strategies that improve conservative therapies are needed. Platelet-rich plasma (PRP) seems to be a good candidate by virtue of its high content of growth factors, most of which are involved in tendon heal...

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Main Authors:	Giusti, Ilaria, D'Ascenzo, Sandra, Mancò, Annalisa, Di Stefano, Gabriella, Di Francesco, Marianna, Rughetti, Anna, Dal Mas, Antonella, Properzi, Gianfranco, Calvisi, Vittorio, Dolo, Vincenza
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132404/

id	pubmed-4132404
recordtype	oai_dc
spelling	pubmed-41324042014-08-21 Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro Giusti, Ilaria D'Ascenzo, Sandra Mancò, Annalisa Di Stefano, Gabriella Di Francesco, Marianna Rughetti, Anna Dal Mas, Antonella Properzi, Gianfranco Calvisi, Vittorio Dolo, Vincenza Research Article Since tendon injuries and tendinopathy are a growing problem, sometimes requiring surgery, new strategies that improve conservative therapies are needed. Platelet-rich plasma (PRP) seems to be a good candidate by virtue of its high content of growth factors, most of which are involved in tendon healing. This study aimed to evaluate if different concentrations of platelets in PRP have different effects on the biological features of normal human tenocytes that are usually required during tendon healing. The different platelet concentrations tested (up to 5 × 106 plt/µL) stimulated differently tenocytes behavior; intermediate concentrations (0.5 × 106, 1 × 106 plt/µL) strongly induced all tested processes (proliferation, migration, collagen, and MMPs production) if compared to untreated cells; on the contrary, the highest concentration had inhibitory effects on proliferation and strongly reduced migration abilities and overall collagen production but, at the same time, induced increasing MMP production, which could be counterproductive because excessive proteolysis could impair tendon mechanical stability. Thus, these in vitro data strongly suggest the need for a compromise between extremely high and low platelet concentrations to obtain an optimal global effect when inducing in vivo tendon healing. Hindawi Publishing Corporation 2014 2014-07-23 /pmc/articles/PMC4132404/ /pubmed/25147809 http://dx.doi.org/10.1155/2014/630870 Text en Copyright © 2014 Ilaria Giusti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Giusti, Ilaria D'Ascenzo, Sandra Mancò, Annalisa Di Stefano, Gabriella Di Francesco, Marianna Rughetti, Anna Dal Mas, Antonella Properzi, Gianfranco Calvisi, Vittorio Dolo, Vincenza
spellingShingle	Giusti, Ilaria D'Ascenzo, Sandra Mancò, Annalisa Di Stefano, Gabriella Di Francesco, Marianna Rughetti, Anna Dal Mas, Antonella Properzi, Gianfranco Calvisi, Vittorio Dolo, Vincenza Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
author_facet	Giusti, Ilaria D'Ascenzo, Sandra Mancò, Annalisa Di Stefano, Gabriella Di Francesco, Marianna Rughetti, Anna Dal Mas, Antonella Properzi, Gianfranco Calvisi, Vittorio Dolo, Vincenza
author_sort	Giusti, Ilaria
title	Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
title_short	Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
title_full	Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
title_fullStr	Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
title_full_unstemmed	Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro
title_sort	platelet concentration in platelet-rich plasma affects tenocyte behavior in vitro
description	Since tendon injuries and tendinopathy are a growing problem, sometimes requiring surgery, new strategies that improve conservative therapies are needed. Platelet-rich plasma (PRP) seems to be a good candidate by virtue of its high content of growth factors, most of which are involved in tendon healing. This study aimed to evaluate if different concentrations of platelets in PRP have different effects on the biological features of normal human tenocytes that are usually required during tendon healing. The different platelet concentrations tested (up to 5 × 106 plt/µL) stimulated differently tenocytes behavior; intermediate concentrations (0.5 × 106, 1 × 106 plt/µL) strongly induced all tested processes (proliferation, migration, collagen, and MMPs production) if compared to untreated cells; on the contrary, the highest concentration had inhibitory effects on proliferation and strongly reduced migration abilities and overall collagen production but, at the same time, induced increasing MMP production, which could be counterproductive because excessive proteolysis could impair tendon mechanical stability. Thus, these in vitro data strongly suggest the need for a compromise between extremely high and low platelet concentrations to obtain an optimal global effect when inducing in vivo tendon healing.
publisher	Hindawi Publishing Corporation
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132404/
_version_	1613124200672788480

Platelet Concentration in Platelet-Rich Plasma Affects Tenocyte Behavior In Vitro

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