Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study
Frutalin is a homotetrameric partly glycosylated α-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biol...
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| Format: | Online |
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Frontiers Media S.A.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126444/ |
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pubmed-41264442014-08-22 Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study Oliveira, Carla Teixeira, José A. Domingues, Lucília Plant Science Frutalin is a homotetrameric partly glycosylated α-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine. Frontiers Media S.A. 2014-08-08 /pmc/articles/PMC4126444/ /pubmed/25152749 http://dx.doi.org/10.3389/fpls.2014.00390 Text en Copyright © 2014 Oliveira, Teixeira and Domingues. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Oliveira, Carla Teixeira, José A. Domingues, Lucília |
| spellingShingle |
Oliveira, Carla Teixeira, José A. Domingues, Lucília Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| author_facet |
Oliveira, Carla Teixeira, José A. Domingues, Lucília |
| author_sort |
Oliveira, Carla |
| title |
Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| title_short |
Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| title_full |
Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| title_fullStr |
Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| title_full_unstemmed |
Recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| title_sort |
recombinant production of plant lectins in microbial systems for biomedical application – the frutalin case study |
| description |
Frutalin is a homotetrameric partly glycosylated α-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variation in isoforms content may lead to inconstant results. The production of lectins by recombinant means has the advantage of obtaining high amounts of proteins with defined amino-acid sequences and more precise properties. In this mini review, we provide the strategies followed to produce two different forms of frutalin in two different microbial systems: Escherichia coli and Pichia pastoris. The processing and functional properties of the recombinant frutalin obtained from these hosts are compared to those of frutalin extracted from breadfruit. Emphasis is given particularly to recombinant frutalin produced in P. pastoris, which showed a remarkable capacity as biomarker of human prostate cancer and as apoptosis-inducer of cancer cells. Recombinant frutalin production opens perspectives for its development as a new tool in human medicine. |
| publisher |
Frontiers Media S.A. |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126444/ |
| _version_ |
1613122457063915520 |