The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils
Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, a...
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Hindawi Publishing Corporation
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124840/ |
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pubmed-41248402014-08-18 The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils Dkhil, Mohamed A. Al-Quraishy, Saleh Al-Khalifa, Mohamed S. Research Article Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, and the presence of oxidative stress. Mongolian gerbils were challenged with 5 × 106 Babesia divergens infected erythrocytes. Parasitemia reached approximately 77% at day 5 postinfection. Infection also induced injury of the spleen. This was evidenced with (i) increases in cellular damage of the spleen, (ii) decrease in antioxidant capacity as indicated by decreased glutathione, catalase, and superoxide dismutase levels, (iii) increased production of malondialdehyde and nitric oxide derived products (nitrite/nitrate), and (iv) increased lactic acid dehydrogenase activity and protein carbonyl content in the spleen. Infection interfered with normal cell cycle of the spleen cells at G0/G1, S, and G2/M phases. On the basis of the above results it can be hypothesized that B. divergens infected erythrocytes could alter the spleen histopathology and cause cell cycle alteration and induce oxidative stress in splenic tissue. Hindawi Publishing Corporation 2014 2014-07-17 /pmc/articles/PMC4124840/ /pubmed/25136591 http://dx.doi.org/10.1155/2014/483854 Text en Copyright © 2014 Mohamed A. Dkhil et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Dkhil, Mohamed A. Al-Quraishy, Saleh Al-Khalifa, Mohamed S. |
| spellingShingle |
Dkhil, Mohamed A. Al-Quraishy, Saleh Al-Khalifa, Mohamed S. The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| author_facet |
Dkhil, Mohamed A. Al-Quraishy, Saleh Al-Khalifa, Mohamed S. |
| author_sort |
Dkhil, Mohamed A. |
| title |
The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| title_short |
The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| title_full |
The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| title_fullStr |
The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| title_full_unstemmed |
The Effect of Babesia divergens Infection on the Spleen of Mongolian Gerbils |
| title_sort |
effect of babesia divergens infection on the spleen of mongolian gerbils |
| description |
Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, and the presence of oxidative stress. Mongolian gerbils were challenged with 5 × 106
Babesia divergens infected erythrocytes. Parasitemia reached approximately 77% at day 5 postinfection. Infection also induced injury of the spleen. This was evidenced with (i) increases in cellular damage of the spleen, (ii) decrease in antioxidant capacity as indicated by decreased glutathione, catalase, and superoxide dismutase levels, (iii) increased production of malondialdehyde and nitric oxide derived products (nitrite/nitrate), and (iv) increased lactic acid dehydrogenase activity and protein carbonyl content in the spleen. Infection interfered with normal cell cycle of the spleen cells at G0/G1, S, and G2/M phases. On the basis of the above results it can be hypothesized that B. divergens infected erythrocytes could alter the spleen histopathology and cause cell cycle alteration and induce oxidative stress in splenic tissue. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124840/ |
| _version_ |
1613121918954635264 |