Prevalence of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran
Background and Objectives. The aim of this study was to determine the frequency of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM type genes among A. baumannii isolates from hospitalized patients in two hospitals in Tehran, Iran. Patients and Methods. Antibiotic susceptibility tests were performed...
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Hindawi Publishing Corporation
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123593/ |
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pubmed-41235932014-08-17 Prevalence of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran Fallah, Fatemeh Noori, Maryam Hashemi, Ali Goudarzi, Hossein Karimi, Abdollah Erfanimanesh, Soroor Alimehr, Shadi Research Article Background and Objectives. The aim of this study was to determine the frequency of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM type genes among A. baumannii isolates from hospitalized patients in two hospitals in Tehran, Iran. Patients and Methods. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and Broth microdilution methods. The frequency of MBL (metallo-beta-lactamase) and ESBL (extended-spectrum-beta-lactamase) producers was evaluated by CDDT. The β -lactamases genes were detected by PCR and sequencing methods. Results. The resistance of A. baumannii isolates against tested antibiotics was as follows: 103 (95.4%) to ceftazidime, 108 (100%) to cefotaxime, 105 (95.7%) to cefepime, 99 (91.7%) to imipenem, 99 (91.7%) to meropenem, 87 (80.6%) to amikacin, 105 (97.2%) to piperacillin, 100 (92.6%) to ciprofloxacin, 103 (95.4%) to piperacillin/tazobactam, 44 (40.7%) to gentamicin, 106 (98.1%) to ampicillin/sulbactam, 106 (98.1%) to co-trimoxazole, 87 (80.6%) to tetracycline, and 1 (1.8%) to colistin. Using combined disk diffusion test, 91 (84.2%) and 86 (86.86%) were ESBL and MBL producers, respectively. The prevalence of bla PER-1, bla VEB-1, bla IMP-1, and bla VIM-1 genes was 71 (78.03%), 36 (39.5%), 3 (3.48%), and 15 (17.44%), respectively. Conclusions. The prevalence of ESBLs and MBLs-producing A. baumannii strains detected in this study is a major concern and highlights the need of infection control measures. Hindawi Publishing Corporation 2014 2014-07-15 /pmc/articles/PMC4123593/ /pubmed/25133013 http://dx.doi.org/10.1155/2014/245162 Text en Copyright © 2014 Fatemeh Fallah et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Fallah, Fatemeh Noori, Maryam Hashemi, Ali Goudarzi, Hossein Karimi, Abdollah Erfanimanesh, Soroor Alimehr, Shadi |
| spellingShingle |
Fallah, Fatemeh Noori, Maryam Hashemi, Ali Goudarzi, Hossein Karimi, Abdollah Erfanimanesh, Soroor Alimehr, Shadi Prevalence of bla NDM, bla PER, bla VEB, bla IMP, and bla VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| author_facet |
Fallah, Fatemeh Noori, Maryam Hashemi, Ali Goudarzi, Hossein Karimi, Abdollah Erfanimanesh, Soroor Alimehr, Shadi |
| author_sort |
Fallah, Fatemeh |
| title |
Prevalence of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| title_short |
Prevalence of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| title_full |
Prevalence of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| title_fullStr |
Prevalence of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| title_full_unstemmed |
Prevalence of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM Genes among Acinetobacter baumannii Isolated from Two Hospitals of Tehran, Iran |
| title_sort |
prevalence of bla
ndm, bla
per, bla
veb, bla
imp, and bla
vim genes among acinetobacter baumannii isolated from two hospitals of tehran, iran |
| description |
Background and Objectives. The aim of this study was to determine the frequency of bla
NDM, bla
PER, bla
VEB, bla
IMP, and bla
VIM type genes among A. baumannii isolates from hospitalized patients in two hospitals in Tehran, Iran. Patients and Methods. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and Broth microdilution methods. The frequency of MBL (metallo-beta-lactamase) and ESBL (extended-spectrum-beta-lactamase) producers was evaluated by CDDT. The β
-lactamases genes were detected by PCR and sequencing methods. Results. The resistance of A. baumannii isolates against tested antibiotics was as follows: 103 (95.4%) to ceftazidime, 108 (100%) to cefotaxime, 105 (95.7%) to cefepime, 99 (91.7%) to imipenem, 99 (91.7%) to meropenem, 87 (80.6%) to amikacin, 105 (97.2%) to piperacillin, 100 (92.6%) to ciprofloxacin, 103 (95.4%) to piperacillin/tazobactam, 44 (40.7%) to gentamicin, 106 (98.1%) to ampicillin/sulbactam, 106 (98.1%) to co-trimoxazole, 87 (80.6%) to tetracycline, and 1 (1.8%) to colistin. Using combined disk diffusion test, 91 (84.2%) and 86 (86.86%) were ESBL and MBL producers, respectively. The prevalence of bla
PER-1, bla
VEB-1, bla
IMP-1, and bla
VIM-1 genes was 71 (78.03%), 36 (39.5%), 3 (3.48%), and 15 (17.44%), respectively. Conclusions. The prevalence of ESBLs and MBLs-producing A. baumannii strains detected in this study is a major concern and highlights the need of infection control measures. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4123593/ |
| _version_ |
1613121514406674432 |