Absolute quantification by droplet digital PCR versus analog real-time PCR

Absolute quantification by droplet digital PCR versus analog real-time PCR

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased by 37–86%) and improved day-...

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Bibliographic Details
Main Authors: Hindson, Christopher M, Chevillet, John R, Briggs, Hilary A, Gallichotte, Emily N, Ruf, Ingrid K, Hindson, Benjamin J, Vessella, Robert L, Tewari, Muneesh
Format: Online
Language:English
Published: 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118677/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118677/

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