Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells
We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, th...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111573/ |
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pubmed-4111573 |
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pubmed-41115732014-07-29 Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells Ji, Debin Wang, Yinsheng Research Article We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells. Public Library of Science 2014-07-25 /pmc/articles/PMC4111573/ /pubmed/25061973 http://dx.doi.org/10.1371/journal.pone.0103335 Text en © 2014 Ji, Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ji, Debin Wang, Yinsheng |
| spellingShingle |
Ji, Debin Wang, Yinsheng Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| author_facet |
Ji, Debin Wang, Yinsheng |
| author_sort |
Ji, Debin |
| title |
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| title_short |
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| title_full |
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| title_fullStr |
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| title_full_unstemmed |
Facile Enzymatic Synthesis of Base J-Containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells |
| title_sort |
facile enzymatic synthesis of base j-containing oligodeoxyribonucleotides and an analysis of the impact of base j on dna replication in cells |
| description |
We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells. |
| publisher |
Public Library of Science |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4111573/ |
| _version_ |
1613118057503260672 |