Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors

Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenc...

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Main Authors:	Krause, Matthew P., Dworski, Shaalee, Feinberg, Konstantin, Jones, Karen, Johnston, Adam P.W., Paul, Smitha, Paris, Maryline, Peles, Elior, Bagli, Darius, Forrest, Christopher R., Kaplan, David R., Miller, Freda D.
Format:	Online
Language:	English
Published:	Elsevier 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110792/

id	pubmed-4110792
recordtype	oai_dc
spelling	pubmed-41107922014-07-25 Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors Krause, Matthew P. Dworski, Shaalee Feinberg, Konstantin Jones, Karen Johnston, Adam P.W. Paul, Smitha Paris, Maryline Peles, Elior Bagli, Darius Forrest, Christopher R. Kaplan, David R. Miller, Freda D. Article Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from neural crest-derived facial and mesodermally derived foreskin dermis and the foreskin SKPs can make myelinating Schwann cells. Thus, nonneural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally defined lineage boundaries are more flexible than widely thought. Elsevier 2014-06-19 /pmc/articles/PMC4110792/ /pubmed/25068124 http://dx.doi.org/10.1016/j.stemcr.2014.05.011 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Krause, Matthew P. Dworski, Shaalee Feinberg, Konstantin Jones, Karen Johnston, Adam P.W. Paul, Smitha Paris, Maryline Peles, Elior Bagli, Darius Forrest, Christopher R. Kaplan, David R. Miller, Freda D.
spellingShingle	Krause, Matthew P. Dworski, Shaalee Feinberg, Konstantin Jones, Karen Johnston, Adam P.W. Paul, Smitha Paris, Maryline Peles, Elior Bagli, Darius Forrest, Christopher R. Kaplan, David R. Miller, Freda D. Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
author_facet	Krause, Matthew P. Dworski, Shaalee Feinberg, Konstantin Jones, Karen Johnston, Adam P.W. Paul, Smitha Paris, Maryline Peles, Elior Bagli, Darius Forrest, Christopher R. Kaplan, David R. Miller, Freda D.
author_sort	Krause, Matthew P.
title	Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_short	Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_full	Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_fullStr	Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_full_unstemmed	Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors
title_sort	direct genesis of functional rodent and human schwann cells from skin mesenchymal precursors
description	Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from neural crest-derived facial and mesodermally derived foreskin dermis and the foreskin SKPs can make myelinating Schwann cells. Thus, nonneural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally defined lineage boundaries are more flexible than widely thought.
publisher	Elsevier
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4110792/
_version_	1613117894559793152

Direct Genesis of Functional Rodent and Human Schwann Cells from Skin Mesenchymal Precursors

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