Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives
A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various ad...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106064/ |
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pubmed-4106064 |
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pubmed-41060642014-08-07 Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives Nirmal, Nilesh P. Laxman, R. Seeta Research Article A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was evaluated. Among the additives tested, glycerol, mannitol, xylitol, sorbitol, and trehalose were found to be very effective in increasing the stability of FAP, which was found to be concentration dependent. Fivefold increase in residual activity of FAP was observed in the presence of trehalose (50%) and sorbitol (50%) at 50°C for 4 h, compared to FAP without additive. Other additives like calcium at 20 mM and 10–15% ammonium sulphate showed lower stability improvement than trehalose and sorbitol. NaCl, MgCl2, K2HPO4, and glycine were found to be poor stabilizers and showed only a marginal improvement. PEG 6000 did not show any increase in stability but was found to be slightly inhibitory. Hindawi Publishing Corporation 2014 2014-07-03 /pmc/articles/PMC4106064/ /pubmed/25105022 http://dx.doi.org/10.1155/2014/109303 Text en Copyright © 2014 N. P. Nirmal and R. S. Laxman. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Nirmal, Nilesh P. Laxman, R. Seeta |
| spellingShingle |
Nirmal, Nilesh P. Laxman, R. Seeta Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| author_facet |
Nirmal, Nilesh P. Laxman, R. Seeta |
| author_sort |
Nirmal, Nilesh P. |
| title |
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| title_short |
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| title_full |
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| title_fullStr |
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| title_full_unstemmed |
Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives |
| title_sort |
enhanced thermostability of a fungal alkaline protease by different additives |
| description |
A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was evaluated. Among the additives tested, glycerol, mannitol, xylitol, sorbitol, and trehalose were found to be very effective in increasing the stability of FAP, which was found to be concentration dependent. Fivefold increase in residual activity of FAP was observed in the presence of trehalose (50%) and sorbitol (50%) at 50°C for 4 h, compared to FAP without additive. Other additives like calcium at 20 mM and 10–15% ammonium sulphate showed lower stability improvement than trehalose and sorbitol. NaCl, MgCl2, K2HPO4, and glycine were found to be poor stabilizers and showed only a marginal improvement. PEG 6000 did not show any increase in stability but was found to be slightly inhibitory. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106064/ |
| _version_ |
1613116460575490048 |