Development of organophosphate hydrolase activity in a bacterial homolog of human cholinesterase
We applied a combination of rational design and directed evolution (DE) to Bacillus subtilis p-nitrobenzyl esterase (pNBE) with the goal of enhancing organophosphorus acid anhydride hydrolase (OPAAH) activity. DE started with a designed variant, pNBE A107H, carrying a histidine homologous with human...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100338/ |