Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification...

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Bibliographic Details
Main Authors: Alias, Norsyuhada, Ahmad Mazian, Mu'adz, Salleh, Abu Bakar, Basri, Mahiran, Rahman, Raja Noor Zaliha Raja Abd.
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100274/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100274/

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