Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens
The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we clone...
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MDPI
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100098/ |
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pubmed-41000982014-07-16 Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens Wang, Baosheng Yu, Jingjuan Zhu, Dengyun Chang, Yujie Zhao, Qian Article The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens. MDPI 2014-05-26 /pmc/articles/PMC4100098/ /pubmed/24865494 http://dx.doi.org/10.3390/ijms15069343 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wang, Baosheng Yu, Jingjuan Zhu, Dengyun Chang, Yujie Zhao, Qian |
| spellingShingle |
Wang, Baosheng Yu, Jingjuan Zhu, Dengyun Chang, Yujie Zhao, Qian Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| author_facet |
Wang, Baosheng Yu, Jingjuan Zhu, Dengyun Chang, Yujie Zhao, Qian |
| author_sort |
Wang, Baosheng |
| title |
Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| title_short |
Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| title_full |
Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| title_fullStr |
Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| title_full_unstemmed |
Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens |
| title_sort |
maize zmrack1 is involved in the plant response to fungal phytopathogens |
| description |
The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens. |
| publisher |
MDPI |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100098/ |
| _version_ |
1613114491951644672 |