Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse

Polychlorinated biphenyls (PCBs) are stable, lipophilic compounds that accumulate in the environment and in the food chain. Though some studies provided evidence that PCBs had adverse effects on reproductive function, most of these results were from in vitro models. Therefore we investigated the eff...

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Main Authors:	Liu, ShuZhen, Jiang, LiGang, Meng, XiaoQian, Han, XiaoYing, Cheng, Dong, Zhang, TianLiang, Miao, YiLiang
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094510/

id	pubmed-4094510
recordtype	oai_dc
spelling	pubmed-40945102014-07-15 Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse Liu, ShuZhen Jiang, LiGang Meng, XiaoQian Han, XiaoYing Cheng, Dong Zhang, TianLiang Miao, YiLiang Research Article Polychlorinated biphenyls (PCBs) are stable, lipophilic compounds that accumulate in the environment and in the food chain. Though some studies provided evidence that PCBs had adverse effects on reproductive function, most of these results were from in vitro models. Therefore we investigated the effect of Aroclor 1254 (a commercial PCBs mixture) treatments on in vivo maturation and developmental potential of mouse oocytes. In the present study, female ICR mice were treated with different doses (12.5, 25 and 50 mg/kg) of Aroclor 1254 (a commercial PCB mixture) once every 72 hours by intraperitoneal injection for 9 days. After three treatments of Aroclor 1254, the mice were superovulated to collect oocytes one day after the last exposure. The effects of Aroclor 1254 on oocyte maturation, fertilization, and preimplantation embryonic development were investigated. Immunofluorescence-stained oocytes were observed under a confocal microscope to assess the effects of Aroclor 1254 on spindle morphology. Parthenogenic activation and the incidence of cumulus apoptosis in cumulus-oocyte complexes were observed as well. Oocytes exposed to different doses of Aroclor 1254 in vivo were associated with a significant decrease in outgrowth potential, abnormal spindle configurations, and the inhibition of parthenogenetic activation of ovulated oocytes. Furthermore, the incidence of apoptosis in cumulus cells was increased after exposed to Aroclor 1254. These results may provide reference for the treatment of reproductive diseases such as infertility or miscarriage caused by environmental contaminants. Public Library of Science 2014-07-11 /pmc/articles/PMC4094510/ /pubmed/25013911 http://dx.doi.org/10.1371/journal.pone.0102064 Text en © 2014 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, ShuZhen Jiang, LiGang Meng, XiaoQian Han, XiaoYing Cheng, Dong Zhang, TianLiang Miao, YiLiang
spellingShingle	Liu, ShuZhen Jiang, LiGang Meng, XiaoQian Han, XiaoYing Cheng, Dong Zhang, TianLiang Miao, YiLiang Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
author_facet	Liu, ShuZhen Jiang, LiGang Meng, XiaoQian Han, XiaoYing Cheng, Dong Zhang, TianLiang Miao, YiLiang
author_sort	Liu, ShuZhen
title	Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
title_short	Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
title_full	Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
title_fullStr	Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
title_full_unstemmed	Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse
title_sort	effects of aroclor 1254 on in vivo oocyte maturation in the mouse
description	Polychlorinated biphenyls (PCBs) are stable, lipophilic compounds that accumulate in the environment and in the food chain. Though some studies provided evidence that PCBs had adverse effects on reproductive function, most of these results were from in vitro models. Therefore we investigated the effect of Aroclor 1254 (a commercial PCBs mixture) treatments on in vivo maturation and developmental potential of mouse oocytes. In the present study, female ICR mice were treated with different doses (12.5, 25 and 50 mg/kg) of Aroclor 1254 (a commercial PCB mixture) once every 72 hours by intraperitoneal injection for 9 days. After three treatments of Aroclor 1254, the mice were superovulated to collect oocytes one day after the last exposure. The effects of Aroclor 1254 on oocyte maturation, fertilization, and preimplantation embryonic development were investigated. Immunofluorescence-stained oocytes were observed under a confocal microscope to assess the effects of Aroclor 1254 on spindle morphology. Parthenogenic activation and the incidence of cumulus apoptosis in cumulus-oocyte complexes were observed as well. Oocytes exposed to different doses of Aroclor 1254 in vivo were associated with a significant decrease in outgrowth potential, abnormal spindle configurations, and the inhibition of parthenogenetic activation of ovulated oocytes. Furthermore, the incidence of apoptosis in cumulus cells was increased after exposed to Aroclor 1254. These results may provide reference for the treatment of reproductive diseases such as infertility or miscarriage caused by environmental contaminants.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094510/
_version_	1613112530767446016

Effects of Aroclor 1254 on In Vivo Oocyte Maturation in the Mouse

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