Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats

Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats

MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goat...

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Main Authors: Ji, Zhibin, Wang, Guizhi, Zhang, Chunlan, Xie, Zhijing, Liu, Zhaohua, Wang, Jianmin
Format: Online
Language:English
Published: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093481/
id pubmed-4093481
recordtype oai_dc
spelling pubmed-40934812014-07-21 Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats Ji, Zhibin Wang, Guizhi Zhang, Chunlan Xie, Zhijing Liu, Zhaohua Wang, Jianmin Article MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2013-03 /pmc/articles/PMC4093481/ /pubmed/25049792 http://dx.doi.org/10.5713/ajas.2012.12422 Text en Copyright © 2013 by Asian-Australasian Journal of Animal Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Ji, Zhibin
Wang, Guizhi
Zhang, Chunlan
Xie, Zhijing
Liu, Zhaohua
Wang, Jianmin
spellingShingle Ji, Zhibin
Wang, Guizhi
Zhang, Chunlan
Xie, Zhijing
Liu, Zhaohua
Wang, Jianmin
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
author_facet Ji, Zhibin
Wang, Guizhi
Zhang, Chunlan
Xie, Zhijing
Liu, Zhaohua
Wang, Jianmin
author_sort Ji, Zhibin
title Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
title_short Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
title_full Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
title_fullStr Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
title_full_unstemmed Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
title_sort identification and function prediction of novel micrornas in laoshan dairy goats
description MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.
publisher Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4093481/
_version_ 1613112264333721600

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