Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer

The transcription factor SOX2 is essential for maintaining pluripotency in a variety of stem cells. It has important functions during embryonic development, is involved in cancer stem cell maintenance, and is often deregulated in cancer. The mechanism of SOX2 regulation has yet to be clarified, but...

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Main Authors:	Askarian-Amiri, Marjan E., Seyfoddin, Vahid, Smart, Chanel E., Wang, Jingli, Kim, Ji Eun, Hansji, Herah, Baguley, Bruce C., Finlay, Graeme J., Leung, Euphemia Y.
Format:	Online
Language:	English
Published:	Public Library of Science 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090206/

id	pubmed-4090206
recordtype	oai_dc
spelling	pubmed-40902062014-07-14 Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer Askarian-Amiri, Marjan E. Seyfoddin, Vahid Smart, Chanel E. Wang, Jingli Kim, Ji Eun Hansji, Herah Baguley, Bruce C. Finlay, Graeme J. Leung, Euphemia Y. Research Article The transcription factor SOX2 is essential for maintaining pluripotency in a variety of stem cells. It has important functions during embryonic development, is involved in cancer stem cell maintenance, and is often deregulated in cancer. The mechanism of SOX2 regulation has yet to be clarified, but the SOX2 gene lies in an intron of a long multi-exon non-coding RNA called SOX2 overlapping transcript (SOX2OT). Here, we show that the expression of SOX2 and SOX2OT is concordant in breast cancer, differentially expressed in estrogen receptor positive and negative breast cancer samples and that both are up-regulated in suspension culture conditions that favor growth of stem cell phenotypes. Importantly, ectopic expression of SOX2OT led to an almost 20-fold increase in SOX2 expression, together with a reduced proliferation and increased breast cancer cell anchorage-independent growth. We propose that SOX2OT plays a key role in the induction and/or maintenance of SOX2 expression in breast cancer. Public Library of Science 2014-07-09 /pmc/articles/PMC4090206/ /pubmed/25006803 http://dx.doi.org/10.1371/journal.pone.0102140 Text en © 2014 Askarian-Amiri et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Askarian-Amiri, Marjan E. Seyfoddin, Vahid Smart, Chanel E. Wang, Jingli Kim, Ji Eun Hansji, Herah Baguley, Bruce C. Finlay, Graeme J. Leung, Euphemia Y.
spellingShingle	Askarian-Amiri, Marjan E. Seyfoddin, Vahid Smart, Chanel E. Wang, Jingli Kim, Ji Eun Hansji, Herah Baguley, Bruce C. Finlay, Graeme J. Leung, Euphemia Y. Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
author_facet	Askarian-Amiri, Marjan E. Seyfoddin, Vahid Smart, Chanel E. Wang, Jingli Kim, Ji Eun Hansji, Herah Baguley, Bruce C. Finlay, Graeme J. Leung, Euphemia Y.
author_sort	Askarian-Amiri, Marjan E.
title	Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
title_short	Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
title_full	Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
title_fullStr	Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
title_full_unstemmed	Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer
title_sort	emerging role of long non-coding rna sox2ot in sox2 regulation in breast cancer
description	The transcription factor SOX2 is essential for maintaining pluripotency in a variety of stem cells. It has important functions during embryonic development, is involved in cancer stem cell maintenance, and is often deregulated in cancer. The mechanism of SOX2 regulation has yet to be clarified, but the SOX2 gene lies in an intron of a long multi-exon non-coding RNA called SOX2 overlapping transcript (SOX2OT). Here, we show that the expression of SOX2 and SOX2OT is concordant in breast cancer, differentially expressed in estrogen receptor positive and negative breast cancer samples and that both are up-regulated in suspension culture conditions that favor growth of stem cell phenotypes. Importantly, ectopic expression of SOX2OT led to an almost 20-fold increase in SOX2 expression, together with a reduced proliferation and increased breast cancer cell anchorage-independent growth. We propose that SOX2OT plays a key role in the induction and/or maintenance of SOX2 expression in breast cancer.
publisher	Public Library of Science
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090206/
_version_	1613110904909463552

Emerging Role of Long Non-Coding RNA SOX2OT in SOX2 Regulation in Breast Cancer

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