Correlated cryogenic photoactivated localization microscopy and electron cryotomography

Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyon...

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Main Authors:	Chang, Yi-Wei, Chen, Songye, Tocheva, Elitza I., Treuner-Lange, Anke, Löbach, Stephanie, Søgaard-Andersen, Lotte, Jensen, Grant J.
Format:	Online
Language:	English
Published:	2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/

id	pubmed-4081473
recordtype	oai_dc
spelling	pubmed-40814732015-01-01 Correlated cryogenic photoactivated localization microscopy and electron cryotomography Chang, Yi-Wei Chen, Songye Tocheva, Elitza I. Treuner-Lange, Anke Löbach, Stephanie Søgaard-Andersen, Lotte Jensen, Grant J. Article Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyond the diffraction limit of the light microscope, and use it to identify multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus. 2014-05-11 2014-07 /pmc/articles/PMC4081473/ /pubmed/24813625 http://dx.doi.org/10.1038/nmeth.2961 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chang, Yi-Wei Chen, Songye Tocheva, Elitza I. Treuner-Lange, Anke Löbach, Stephanie Søgaard-Andersen, Lotte Jensen, Grant J.
spellingShingle	Chang, Yi-Wei Chen, Songye Tocheva, Elitza I. Treuner-Lange, Anke Löbach, Stephanie Søgaard-Andersen, Lotte Jensen, Grant J. Correlated cryogenic photoactivated localization microscopy and electron cryotomography
author_facet	Chang, Yi-Wei Chen, Songye Tocheva, Elitza I. Treuner-Lange, Anke Löbach, Stephanie Søgaard-Andersen, Lotte Jensen, Grant J.
author_sort	Chang, Yi-Wei
title	Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_short	Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_full	Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_fullStr	Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_full_unstemmed	Correlated cryogenic photoactivated localization microscopy and electron cryotomography
title_sort	correlated cryogenic photoactivated localization microscopy and electron cryotomography
description	Electron cryotomography (ECT) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated "cryo-PALM"-ECT method for localizing objects within cryotomograms to beyond the diffraction limit of the light microscope, and use it to identify multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081473/
_version_	1613108041851338752

Correlated cryogenic photoactivated localization microscopy and electron cryotomography

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