CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

CRISPR/Cas9 systems are a versatile tool for genome editing due to the highly efficient targeting of DNA sequences complementary to their RNA guide strands. However, it has been shown that RNA-guided Cas9 nuclease cleaves genomic DNA sequences containing mismatches to the guide strand. A better unde...

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Bibliographic Details
Main Authors: Lin, Yanni, Cradick, Thomas J., Brown, Matthew T., Deshmukh, Harshavardhan, Ranjan, Piyush, Sarode, Neha, Wile, Brian M., Vertino, Paula M., Stewart, Frank J., Bao, Gang
Format: Online
Language:English
Published: Oxford University Press 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066799/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066799/

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